角化细胞生长因子的获得及植物表达载体的构建  

Cloning of Recombinant Keratinocyte Growth Factor and Its Construction of Plant Expression Vector

作　　者：邵寒娟[1] 林涛[1] 苏勇波[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2004年第1期124-127,共4页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金(B0210002)资助(2002年)

摘　　要：从鼠肺中提取RNA,设计相应的引物,经RT PCR扩增出角化细胞生长因子cDNA序列;插入pMD18 T载体,经DNA测序证实为KGF基因.将KGF基因经酶切后连接于植物CaMV35S启动子和NOS终止子之间,单酶切插入pBI1301植物双元表达载体,转化大肠杆菌,阳性克隆提取质粒后转化农杆菌EHA105,实现KGF植物表达载体的构建,为KGF的进一步研究奠定基础.Abstract: The cDNA gene encoding keratinocyte growth factor (KGF) was amplified from mouse lung by RT-PCR and inserted into pMD18-T vector. The result of automatic sequence showed that the sequence of inserted fragment was KGF gene. The KGF gene was inserted into pBPFΩ7 under the control of CaMV35S promotor and NOS terminator, then was digested and inserted into pBI 1301 to form the plant binary expression vector. The plant binary expression vector of KGF was transformed into Agrobacterium EHA 105. It provided a reliable basis for the further study on KGF.

关 键 词：角化细胞生长因子 基因克隆 植物表达载体 KGF基因 有丝分裂剂 

分 类 号：Q253[生物学—细胞生物学] Q782