豇豆胰蛋白酶抑制剂基因转化芥菜及抗虫鉴定  

Transforming Brassica juncea Coss with Cowpea Trypsin Inhibitor Gene and Its Insect-resistance Identification

作　　者：杨朝辉[1] 宋洪元[2] 何凤田[1] 

机构地区：[1]第三军医大学生物化学与分子生物学研究室,重庆400038 [2]西南农业大学园艺系,重庆400076

出　　处：《中国生物化学与分子生物学报》2003年第6期731-735,共5页Chinese Journal of Biochemistry and Molecular Biology

基　　金：重庆市科技攻关课题 (No .98 5 0 84)~~

摘　　要：用农杆菌介导将豇豆胰蛋白酶抑制剂 (CpTI)基因导入芥菜 ,获得了Kan抗性植株 .经PCR扩增、PCR Southern印迹和Northern印迹分析 ,转化再生植株大部分呈阳性 ,而非转化的再生植株均为阴性 ,证明CpTI基因已存在于芥菜基因组中 .在室内进行了喂虫试验 ,结果表明转基因芥菜抗虫性明显高于对照 。Explants of mustard were transformed with Agrobacterium tumefaciens (LBA4404). The putative transformant regeneration plants were assayed by PCR, PCR Southern blot and Northern blot analysis. By these analyses the target bands were observed, the integration of the cowpea trypsin inhibitor(CpTI)gene into mustard genome DNA was confirmed. The result of insect resistance showed that the transgenic plants were more resistant than non transgenic plants.

关 键 词：豇豆胰蛋白酶抑制剂基因 转化 芥菜 抗虫鉴定 农杆菌介导 

分 类 号：S637[农业科学—蔬菜学]