皮下注射和口服螨制剂脱敏在调节小鼠气道炎症和IgE中作用的比较  

Comparison on the Effects of Subcutaneous Injection and Oral Administration of Dust Mite Desensitization Preparations for the Regulation of Airway Inflammation and Serum Level of IgE in Mice

作　　者：吴祖群[1] 许以平[1] 李凝[1] 向华[2] 潘小平[3] 

机构地区：[1]上海第二医科大学附属仁济医院呼吸科,上海200001 [2]复旦大学附属肿瘤医院病理科,上海200032 [3]复旦大学上海医学院生化教研室,上海200032

出　　处：《上海免疫学杂志》2003年第6期400-402,共3页Shanghai Journal of Immunology

摘　　要：为了比较皮下注射和口服螨制剂脱敏在调节小鼠气道炎症和血清IgE中的作用。建立粉尘螨主要过敏原 (Df2 )致BALB/c小鼠气道炎症模型 ,以不同剂量的Df2经皮下注射和口服脱敏 ,检测小鼠支气管肺泡灌洗液 (BALF )中炎症细胞总数及分类、气道炎症细胞浸润程度、小鼠血清总IgE及Df2特异性IgE。结果 :皮下注射组和口服组BALF中炎症细胞总数及嗜酸粒细胞百分比较模型组明显降低 ,气道炎症细胞浸润有所减轻 ,皮下注射组和口服组小鼠血清总IgE均明显降低 ,皮下注射组Df2特异性IgE吸光度值明显降低 ,口服组Df2特异性IgE吸光度值与模型组无明显差异。本实验显示皮下注射和口服脱敏均可减轻该模型小鼠气道炎症细胞浸润 ,皮下注射降低特异性IgE的作用优于口服脱敏。To compare the effects of subcutaneous injection and oral administration of dust mite desentization preparations for the regulation of airway inflammation and serum level of IgE in mice,an airway inflammation animal model in BALB/c mice was established by immunization and challege with major allergen (Df2) of dust mite,and the animals were desensitized with different dosages of Df2 by subcutaneous injection or oral administration. The total amount and different classes of inflammatory cells in the broncho-alveolar lavage fluid (BALF),degree of inflammatory cell infiltration in airway and serum total IgE as well as Df2-specific IgE were determined with suitable methods. The results showed that the total numbers of inflammatory cells and the percentage of eosinophil in BALF were reduced in these two groups of experiments,infiltration of inflammatory cells was also alleviated. The serum total IgE was reduced significantly in these two groups,and the Df2-specific IgE was reduced significantly in the subcutaneous injection group,but not in the oral administration group. These results indicate that both the subcutaneous injection and oral administration methods of desensitization can alleviate the airway inflammation in the BALB/c mice model,yet the subcutaneous injection method is more efficient in reducing the specific IgE than the oral desensitization route.

关 键 词：脱敏 气道炎症 IGE 

分 类 号：R593.1[医药卫生—内科学]