HPLC法测定颈痛灵药酒中大黄素的含量  

Determination of Emodin in Jingtongling medicinal liquor

作　　者：赵春香[1] 刘震凌[1] 王丽娜[1] 王明慧 

机构地区：[1]吉林省药品检验所,长春130062 [2]吉林省天诚药业股份有限公司,长春130102

出　　处：《中国药品标准》2003年第5期46-48,共3页Drug Standards of China

摘　　要：目的:建立颈痛灵药酒的含量测定方法。方法:采用HPLC法,以甲醇—0.1％磷酸溶液(75:25)为流动相,检测波长为436nm。结果:颈癌灵药酒中大黄素的浓度线性范围为2.2～22μg/ml,r=0.9998,平均加样回收率为99.9％,RSD为0.86％(n=5)。结论:该法可作为制剂的含量测定方法。To establish the determination of emodin in Jingtongling medicinal liquor. Methods:HPLC method was used,in methanol-0. 1% phosphoric acid(75:25) as a mobile phase ;detection wavelength at 436nm. Results:The linear range of emodin in Jingtongling medicinal liquor was 2. 2-22μg/ml ,r = 0. 9998. The average recovery of sample was 99. 9% and RSD was 0. 86%(n = 5). Conclusion:The method can be used for content determination of emodin in Jingtongling medicinal liquor.

关 键 词：颈痛灵药酒 大黄素 含量测定 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]