Effect of Ciprofloxacin on the Immune Response of Growing Piglets to Streptococcosis suis  

作　　者：QING Liu ting and YUAN Zong hui (Institute of Veterinary Pharmaceuticals, Huazhong Agricultural University, Wuhan 430070, P. R. China) 

出　　处：《Agricultural Sciences in China》2003年第9期1041-1047,共7页中国农业科学（英文版）

基　　金：theNationalNaturaScienceFoundation (395 70 5 5 5 )

摘　　要：seven week old hybrid healthy pigs (weighted 25.5±1.4 kg ) from a source were randomly allotted to four groups (six each): the blank group, the negative group, the positive group and the test group. Pigs in the negative group and the test group were developed into a typical subacute Streptococcosis suis by inoculating subcutaneously 0.45 billion of pure living Streptococcus suis (type C 55126 ) per kilogram body weight. Pigs in the positive group and the test group were intravenously injected ciprofloxacin (5 mg kg 1 of BW) for 8 consecutive days (twice daily). Pigs in the blank group and positive group were inoculated with a placebo ( 0.85% NaCl). Some immunity parameters were tested on day 0, 7, 14, 21 and 28 after inoculation. Total leukocytes were counted with microscope. Differential leukocyte counts were performed on Giemsa stained blood smears. The effect on the nitroblue tetrazolium (NBT) reduction of neutrophil was used to determine its phagocytic ability. Lymphocyte proliferation was determined by using the mitogens PHA. The total serum IgG concentration was measured by radial single immunodiffusion. A 50% hemolytic test was used to investigate the complement activity in serum. Whereas, a phytohemagglutinin (PHA) skin test was used to investigate in vivo immunity. We got the following results: Ciprofloxacin can retard the clinical signs of an increase of leukocyte counts, a large percent of lymphocytes and a relative percentage decrease of neutrophis. Ciprofloxacin could promote nitroblue tetrazolium (NBT) reduction by neutrophil. Though there was no obvious difference ( P > 0.05 ) between the test group and the negative group, the interaction of both Streptococcosis suis and ciprofloxacin was significantly different( P <0.01, on day 14, 21, 28 post inoculation). The two factors enhanced NBT reduction by neutrophils of pigs in the test group in coordination ( P <0.01, significantly higher than the blank group). In vitro proliferation to phytohemagglutinin (PHA) oseven week old hybrid healthy pigs (weighted 25.5±1.4 kg ) from a source were randomly allotted to four groups (six each): the blank group, the negative group, the positive group and the test group. Pigs in the negative group and the test group were developed into a typical subacute Streptococcosis suis by inoculating subcutaneously 0.45 billion of pure living Streptococcus suis (type C 55126 ) per kilogram body weight. Pigs in the positive group and the test group were intravenously injected ciprofloxacin (5 mg kg 1 of BW) for 8 consecutive days (twice daily). Pigs in the blank group and positive group were inoculated with a placebo ( 0.85% NaCl). Some immunity parameters were tested on day 0, 7, 14, 21 and 28 after inoculation. Total leukocytes were counted with microscope. Differential leukocyte counts were performed on Giemsa stained blood smears. The effect on the nitroblue tetrazolium (NBT) reduction of neutrophil was used to determine its phagocytic ability. Lymphocyte proliferation was determined by using the mitogens PHA. The total serum IgG concentration was measured by radial single immunodiffusion. A 50% hemolytic test was used to investigate the complement activity in serum. Whereas, a phytohemagglutinin (PHA) skin test was used to investigate in vivo immunity. We got the following results: Ciprofloxacin can retard the clinical signs of an increase of leukocyte counts, a large percent of lymphocytes and a relative percentage decrease of neutrophis. Ciprofloxacin could promote nitroblue tetrazolium (NBT) reduction by neutrophil. Though there was no obvious difference ( P > 0.05 ) between the test group and the negative group, the interaction of both Streptococcosis suis and ciprofloxacin was significantly different( P <0.01, on day 14, 21, 28 post inoculation). The two factors enhanced NBT reduction by neutrophils of pigs in the test group in coordination ( P <0.01, significantly higher than the blank group). In vitro proliferation to phytohemagglutinin (PHA) o

关 键 词：PIG CIPROFLOXACIN Streptococcosis suis Immune response 

分 类 号：S828[农业科学—畜牧学]