雌激素对雌性大鼠心内神经节细胞中NOS_1表达的影响  

The Effects of Estrogen on the Expression of NOS_1 in the Intracardiac Ganglionic Neurons of the Female Rats

作　　者：陈小武[1] 祝善乐[2] 范玉华[2] 赵小明[2] 

机构地区：[1]咸宁学院医学院人体解剖学教研室,湖北咸宁437100 [2]华中科技大学同济医学院解剖系

出　　处：《咸宁学院学报（医学版）》2003年第5期314-317,共4页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的探讨雌激素对大鼠心内神经节细胞中NOS1表达的影响。方法本实验建立了去卵巢和雌激素替代疗法动物模型 ,正常饮食饲养 2个月后处死大鼠 ,然后用免疫组化结合图象分析方法观察大鼠心内神经节细胞中NOS1的变化。结果去卵巢大鼠心内神经节细胞中NOS1的表达较正常对照组明显减弱 ,雌激素替代治疗组大鼠心内神经节中NOS1的表达与正常对照组相比无显著性差异 (P >0 .0 5 ) ,而较卵巢摘除组显著增高 ;OVX +TAM +E2组大鼠心内神经节中NOS1的表达较正常对照组显著减弱 ,但与OVX组无显著性差异 (P >0 .0 5 )。Objective To investigate the effects of estrogen on the expression of NOS1 in the intracardiac ganglionic neurons of the female rats.Methods OVX and ERT models were set up.The rats were killed after 2 months.The changes of NOS 1 in intracardiac ganglionic neurons was investigated by immunohistochemical method together with image analysis method.Results The NOS 1 levels in the OVX group were evidently lower than those in the normal group;and there was not evident defference between the ERT group and the normal group;but the NOS 1 levels in the OVX + TAM + E2 group were lower than those in the normal group;there was not evident defference between the OVX group and the OVX + TAM + E2 group.Conclusion The results suggest that estrogen can upregulate the expression of NOS 1 in the cardiac ganglionic neurons of the rats;and then indirectly regulate the functional activities of the cardiovascular system.

关 键 词：雌激素 心内神经节细胞 I型一氧化氮合酶 雌性大鼠 

分 类 号：R977.12[医药卫生—药品]