IL-10 polymorphism is associated with increased incidence of severe sepsis  

作　　者：舒强 方向明 陈齐兴 Frank Stuber 

机构地区：[1]Department of Cardiovascular Surgery, Children's Hospital, Medical College of Zhejiang University, 310016 Hangzhou, China [2]Department of Anaesthesiology, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, 310016 Hangzhou, China [3]Department of Anaesthesiology and Intensive Care Medicine, University of Bonn, Germany

出　　处：《Chinese Medical Journal》2003年第11期1756-1759,共4页中华医学杂志（英文版）

基　　金：a grant from the National Natural Science Foundation of China (No. 30000155).

摘　　要：Objective To investigate whether three biallelic polymorphisms at positions -592, -819 and -1082 in the promoter region of the IL-10 gene are associated with increased incidence of severe sepsis.Methods The IL-10 -592, -819 and -1082 polymorphisms were typed using polymerase chain reaction followed by digestion with the restriction enzymes Rsa I , Mae III and Mnl | , respectively.Results Patients with severe sepsis were more likely to have IL-10 -1082 allele 1, compared with controls (P<0. 05). Genotype distribution of the IL-10 -1082 polymorphism significantly differed between patients and controls (P<0. 05). However, the allele frequencies and genotype distribution of the IL-10 -1082 polymorphism did not differ between surviving and dead patients (P>0. 05). No significant differences in the genotype distribution and allele frequencies of the IL-10 -592 and IL-10 -819 polymorphisms were observed between patients with severe sepsis and heathy controls, nor between surviving and dead patients (P> 0. 05).Conclusions The polymorphism at position -1082 in the promoter region of the IL-10 gene may be associated with susceptibility to severe sepsis. In constrast, the other two highly linked IL-10 polymorphisms are not associated with incidence or the outcome of severe sepsis.Objective To investigate whether three biallelic polymorphisms at positions -592, -819 and -1082 in the promoter region of the IL-10 gene are associated with increased incidence of severe sepsis.Methods The IL-10 -592, -819 and -1082 polymorphisms were typed using polymerase chain reaction followed by digestion with the restriction enzymes Rsa I , Mae III and Mnl | , respectively.Results Patients with severe sepsis were more likely to have IL-10 -1082 allele 1, compared with controls (P<0. 05). Genotype distribution of the IL-10 -1082 polymorphism significantly differed between patients and controls (P<0. 05). However, the allele frequencies and genotype distribution of the IL-10 -1082 polymorphism did not differ between surviving and dead patients (P>0. 05). No significant differences in the genotype distribution and allele frequencies of the IL-10 -592 and IL-10 -819 polymorphisms were observed between patients with severe sepsis and heathy controls, nor between surviving and dead patients (P> 0. 05).Conclusions The polymorphism at position -1082 in the promoter region of the IL-10 gene may be associated with susceptibility to severe sepsis. In constrast, the other two highly linked IL-10 polymorphisms are not associated with incidence or the outcome of severe sepsis.

关 键 词：interleukin-10 ·polymorphism · sepsis 

分 类 号：R631[医药卫生—外科学]