中国人血小板膜糖蛋白Ibα基因VNTR多态性与急性冠脉综合征的关系  

The Relationship between the VNTR Polymorphism in Platelet Glycoprotein Iba Receptor and Acute Coronary Syndromes with or without Ischaemic Events in Chinese Population

作　　者：于泓[1] 倪永斌[1] 胡大一[1] 

机构地区：[1]北京大学人民医院心内科,北京100044

出　　处：《中国医药导刊》2003年第6期411-413,共3页Chinese Journal of Medicinal Guide

摘　　要：目的:探讨血小板GP Ibα基因VNTR多态性与急性冠脉综合征(ACS)的关系。方法:入选135例经冠状动脉造影及临床诊断为ACS患者,PCR扩增目的DNA,采用SDS-PAGE进行VNTR多态性检测,与对照组比较有无差异。135例中39例住院期间发生心脏缺血事件,比较有事件组和无事件组VNTR多态性。结果:①VNTR多态性存在5种基因型:AB、AC、BB、BC和CC,未发现含D等位基因的个体;②基因型肺(AB+AC)和BC在ACS组中较对照组多见,但两组间无显著性差异。A等位基因频率在ACS组有较高趋势(OR:1.56),但与对照组无统计学差异。多因素Logistic回归分析发现5种基因型和3种等位基因的OR值无显著性意义;③发生院内缺血事件组AB基因型较无事件组偏多,但两组间无显著性差异。多因素逐步logistic回归分析发现各基因型不是影响缺血事件的独立变量。根据OR值可以发现重复序列数越多的等位基因,其频率在有事件组就越高的趋势(OR_C:0.72;OR_B:1.33;OR_A:1.50)但两组间无统计学差异。结论:血小板GPIbα巨糖肽区域的VNTR基因多态性与ACS间无显著相关性,对中国人ACS患者住院期间联合终点的发生无显著影响。Objective:. To examine the relationship between the polymorphism of variant number of tandem repeats (VNTR) in the platelet receptor glycoprotein (GP) Ibα and ACS and assess its impact on the occurrence of ischaemic events although with anti-platelet treatment in Chinese Population. Methods: We conducted a case-control study of 135 patients presenting with acute coronary syndromes and 52 inpatient controls without known heart disease. All subjects were examined by coronary angiography and genotyped for VNTR polymorphism in the gene for GP Ibf33 through polymerase chain reaction (PCR) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Results: Binary logistic regression analysis showed the frequency of VNTR polymorphism in the macroglycopeptide region did not significantly differ between ACS patients and control subjects,so did the distribution of A, B or C alleles. When patients with acute coronary syndromes only were analyzed, there was no effect of the number of A, B or C alleles on composite endpoint frequency among Chinese subjects. Conclusions: Our findings indicate that no association exists between the frequency of the polymorphism of VNTR and ACS and a possible association with ischaemic events in Chinese Population.

关 键 词：中国人 血小板 急性冠脉综合征 ACS 糖蛋白Ibα 基因多态性 血栓 

分 类 号：R541.4[医药卫生—心血管疾病]