反相液相色谱法分离2-(4-羟基苯氧基)丙酸酯对映体  

Separation of Enantiomers of 2- (4- hydroxyphenoxy) propionates by Reversed- Phase High- Performance Liquid Chromatography

作　　者：李兵[1] 施介华[2] 江峰[2] 

机构地区：[1]浙江林学院理学院,浙江临安311300 [2]浙江工业大学药学院,浙江杭州310032

出　　处：《分析测试技术与仪器》2003年第4期208-211,共4页Analysis and Testing Technology and Instruments

摘　　要：合成了三-(4-甲基苯甲酸)纤维素酯(MCTB)手性固定相,用反相高效液相色谱法在该手性固定相上对2-(4-羟基苯氧基)丙酸酯对映体进行拆分.实验结果表明,在三-(4-甲基苯甲酸)纤维素酯手性固定相上,以甲醇与水的体积分数为75∶25做流动相能较好的拆分2-(4-羟基苯氧基)丙酸甲酯、乙酯和丁酯对映体,其分离因子分别为1.38、1.49、0.98;同时还发现2-(4-羟基苯氧基)丙酸酯中酯基团的大小对其对映体的分离也有明显的影响,其中以乙酯的拆分效果最佳.Tri - (4- methylbenzoate) cellulose chiral stationary phase (MCTB) was prepared. The separation of enantiomers of 2- (4- hydroxyphenoxy) propionate had been investigated by reversed- phase high- performance liquid chromatography on the chiral stationary phase. The results showed that the separation of enantiomers of methyl, ethyl and butyl esters of 2- (4- hydroxyphenoxy) propionic acid could be achieved on the chiral stationary phase (MCTB) using methanol and water(volume ratio is 75 ∶25) as mobile phase. Their separation factors were 1.38, 1.49, 0.98, respectively. At the same time,It was found that the size of the ester group obviously affected the separation efficiency of their enantiomers on the MCTB chiral stationary phase. The separation of the ethyl ester was most satisfactory.

关 键 词：反相液相色谱法 分离 2-(4-羟基苯氧基)丙酸酯 对映体 手性固定相 除草剂 

分 类 号：TQ457.2[化学工程—农药化工] TQ450.7