重组CHO细胞HBsAg纯化工艺的优化  

Optimizing the Recombinant CHO HBsAg Purification

作　　者：李德娟[1] 陈万革[1] 赵丽剑[1] 江波[1] 赵睿[1] 王智宇[1] 谷丽娟[1] 何巍[1] 

机构地区：[1]长春生物制品研究所,吉林长春130062

出　　处：《生物技术》2003年第6期44-46,共3页Biotechnology

摘　　要：目的 :优化重组CHO细胞HBsAg纯化工艺。方法 :由乙肝病毒S基因转化的中国仓鼠卵巢 (CHO)细胞培养收液 ,经初步提纯、密度梯度离心、凝胶过滤层析可得到HBsAg纯品。结果 :通过凝胶过滤层析收取HBsAg活性峰 ,控制HBsAg活性峰的收量 ,并把HBsAg活性峰的下降段再收集起来重新层析 ,改进后可使HBsAg的总回收率达到 6 0 %以上 ,而且牛血清蛋白残余量达到 10ng ml以下 ,HBsAg纯度 97%以上。结论 :重组CHO细胞HBsAg纯化工艺改进后 ,使HBsAg在产量及质量上均有明显提高。Objective:To optimize the method of recombinant CHO HBsAg purification.Methods:The CHO cells transformed by HBV S-gene were cultured,the cell culture supernatant contained HBsAg was then purified by ammonium sulfate precipitation,density gradient centrifugation,gel filtration,and pure HBsAg was obtained.The fractionation volume was adjusted manually,the HBsAg volume was decreased,and the remained HBsAg peak was recovered,and applied to gel filtration column again.Results:The total recovery of HBsAg could reach up to more than 60%.Residual calf serum was less than 10 ng/ml.HBsAg purity was more than 97%.Conclusion:The Recovery of HBsAg was increased,and the quality of HBsAg was improved simultaneously.

关 键 词：重组CHO细胞 HBSAG 纯化工艺 基因工程 凝胶过滤层析 卵巢细胞 乙肝疫苗 

分 类 号：TQ464[化学工程—制药化工]