编码锌指蛋白的人类新基因TFL76的电子克隆  

In Silicon Cloning of TFL76,A Novel Human Gene Encoding a Zinc Finger Protein

作　　者：李慧[1] 李亦平[1] 慈宏亮[1] 

机构地区：[1]北京师范大学生物医学研究所,北京100875

出　　处：《生物技术》2003年第6期15-17,共3页Biotechnology

摘　　要：目的 :根据基因同源同功原理电子克隆人类新基因。方法 :利用基于基因识别软件Genescan和EST拼接的同源基因克隆法得到人类新基因序列TFL76 ,再利用生物信息学数据库和软件对其进行功能的预测和分析。结果 :TFL76的cDNA序列长2 2 6 8bp ,开放阅读框编码 6 77个氨基酸残基 ,含 12个连续的C2 H2 型锌指基序 ,其分子量为 76kDa。编码区序列被 4个内含子分割。染色体定位于 19q13.4。此位点存在很多与胃癌、膀胱癌、乳腺癌等癌症相关的基因。TFL76的N末端含有多种蛋白激酶的磷酸化位点和核定位信号。结论 :TFL76可能是一个和癌症相关的核转录因子。Objective:In silicon cloning of novel human genes based on the principal that orthologue genes have the alike function.Methods:The novel human gene TFL76 by virtue of orthologue gene cloning based on the gene-finding software Genescan and the EST assembly tools,then take use of the bioinformatical databases and softwares to analyze and predict its function.Results:the cDNA length of TFL76 is 2268b long and a single open reading frame encoding 677 amino acid residues with calculated molecular mass of 76kDa.Analysis of the genome sequence revealed the presence of 4 introns interrupting the coding region.It has many potential protein kinase phosphorylation sites and a nucleus localization signal together with a DNA binding activity conferred by 12 C_2H_2 zinc fingers.TFL76 is mapped to the chromosome of 19q13.4,a region associated with the stomach cancer,the bladder cancer and the breast cancer.Conclusion:TFL76 may be a nuclear transcription factor,which might have roles in human cancer.

关 键 词：电子克隆 锌指蛋白 核转录因子 基因TFL76 人类 

分 类 号：Q987[生物学—遗传学]