影响体细胞核移植效率的因素  

Factors of Affecting the Efficiency of Somatic Nuclear Transfer

作　　者：刘亚[1] 章孝荣[1] 

机构地区：[1]安徽农业大学畜牧水产学院,安徽合肥230036

出　　处：《动物医学进展》2003年第6期35-38,共4页Progress In Veterinary Medicine

基　　金：安徽省"十五"重大科技专项 (0 16 0 30 0 6 ) ;安徽省教育厅自然基金项目 (2 0 0 3kj132 )

摘　　要：哺乳动物体细胞核移植 (克隆 )是近年来迅速发展起来的一项新技术 ,对濒危动物保护、优良种畜快速扩群以及核质关系的研究、药物试验等诸多领域都具有重大意义。但体细胞核移植是一项程序繁杂的工作 ,每一步操作所采用的试剂、材料和方案等都会影响到核移植效率 ,因而其影响因素十分众多。文章根据目前已有资料 ,论述了卵母细胞来源、卵母细胞与供体细胞的胞质容量、卵母细胞的去核方案、体细胞供体动物的年龄、供体细胞的组织来源与传代次数及供体细胞的冷藏、冷冻和所处的细胞周期阶段对核移植效率的影响。另外 ,对重组胚的融合 -激活方案、核移植方案及重构胚的培养方案对核移植效率的影响也作了简要论述。Somatic nuclear transfer in mammalian (clone) is a new technique developed recently. And it is of great value to protecting those dying out animal species, increasing the number of excellent livestock, studying the relation between nucleus and oocytoplasm and pharmacology study. For being a complex engineering, the efficiency of nuclear transfer is affected by so many factors, such as the regents, materials and program employed in the experiment. In this paper, the effect on nuclear transfer from resource of oocyte, the volume of oocyte and donor cell, the method of enucleating, age of somatic cell donor, histological origin and passage number of donor cell, the phase of donor cell-cycle were discussed. Moreover, the scheme of fusion-activation of reconstituted oocyte, nuclear transfer program and culture method of reconstructed oocyte were related, too.

关 键 词：体细胞核 移植效率 影响因素 卵母细胞 胞质容量 动物育种 

分 类 号：S813[农业科学—畜牧学]