扫描及负染电镜在兔脑炎原虫病诊断中的应用  

Use of the Scanning and Negative Staining Electron Microscopy in Diagnosing Encephalitozoonosis

作　　者：张柳平[1] 段艳[1] 潘耀谦[1] 夏志平[1] 邹啸环[1] 

机构地区：[1]解放军军需大学军事兽医系,吉林长春130062

出　　处：《动物医学进展》2003年第6期96-97,共2页Progress In Veterinary Medicine

基　　金：国家自然科学基金资助项目 (39970 5 6 6 )

摘　　要：12只临床表现头颈歪斜、昏睡、震颤、局部或全身轻瘫的可疑为兔脑炎原虫病的獭兔 ,对其肾脏皮髓交界和尿沉渣分别进行了扫描电镜和负染电镜观察 ,看到了大小为 1～ 1 .5× 1 .4～ 2 .5μm,形态呈卵圆形和杆状的兔脑炎原虫。阳性检出率为 1 0 0 %。说明扫描及负染电镜术是诊断兔脑炎原虫病的一种较为可靠的技术。otter rabbits with head tilt,lethargy,tremor,partly or total body paralysis were suspected as encephalitozoonosis. The locations between cortex and medulla and urinary sediments were examined by scanning and negative staining electron microscopy.Encephalitozoon cuniculus were elongated and oval in shapes and 1~1.5 μm×1.4~2.5 μm in sizes under electron microscopy. All rabbits were diagnosed as encephalitozoonosis. It was thought that the scanning and negative staining electron microscopy method was a reliable method to identify encephalitozoonosis.

关 键 词：扫描 负染电镜 兔脑炎 原虫病 诊断 临床表现 阳性检出率 

分 类 号：S858.291[农业科学—临床兽医学]