用PCR对鸡毒支原体感染的检测  

Detection MG of Artificial Infection and Clinical Samples by Polymerase Chain Reaction

作　　者：高以明[1] 李建[2] 朱秉权[2] 

机构地区：[1]连云港出入境检验检疫局,江苏连云港222042 [2]南通出入境检验检疫局,江苏南通226005

出　　处：《动物医学进展》2003年第6期84-85,共2页Progress In Veterinary Medicine

摘　　要：应用已建立的检测 MG和 MS的 PCR方法 ,对人工接种鸡毒支原体后 2～ 2 0 d的 SPF鸡气管棉拭样品和气管、肺、肝、脾、胸肌、腿肌等器官和组织进行了检测 ;对现场采集的样品同样作 PCR检测。结果表明 ,上述人工样品均检测到病原 ,以气管棉拭样品检出最多 ;现场样品PCR的阳性检出率为 1 0 .2 8% ,分离培养的阳性率为 2 .8% ,敏感性前者高于后者。SPF chickens were infected with MG by inoculation of trachea. The samples of trachea swab, trachea, lung,liver,spleen, chest muscle and leg muscle in SPF chickens were collected from 2 and 20 days postinoculation. The clinical samples were also collected. MG and MS in all sample were detected by PCR. Results showed that MG positive rate in the trachea swab of artificial samples is more than that in other tissues. The MG positive rate detected by PCR in clinical samples is 10.28%, the MG positive rate detected by culture method is 2.8%.

关 键 词：PCR 鸡毒支原体 感染率 检测技术 人工接种 阳性检出率 

分 类 号：S858.31[农业科学—临床兽医学]