组织工程血管脱细胞支架主要组织相容性复合体Ⅰ抗原性的免疫组化观察  

Immunohistochemical observation of major histocompatibility complex class Ⅰ ant igenicity in decellularized scaffolds of tissue engineered blood vessels

作　　者：刘太华[1] 张炎[1] 姜宗来[2] 李玉泉[1] 刘波[1] 张秀花[1] 

机构地区：[1]第二军医大学基础医学部人体解剖学教研室,上海200433 [2]上海交通大学医学院,上海200030

出　　处：《第二军医大学学报》2004年第1期72-74,F004,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金 ( 10 2 72 110 ;10 13 2 0 2 0 )

摘　　要：目的 :探讨组织工程血管全生物支架的免疫原性的检测方法。 方法 :取猪颈总动脉 ,剥离外膜 ,剪成 2 cm长的血管段 ,随机分为 3组 :对照组、脱细胞组和脱细胞加碱处理组 ,每组 5段。以免疫组化方法检测血管脱细胞处理前后主要组织相容性复合体 (MHC )的变化情况 ,并将检测结果作图像分析。 结果 :对照组血管壁 MHC 高表达 ,脱细胞处理后 MHC 明显减少。图像分析得出阳性颗粒面积与视场面积比 :对照组为 (2 4 .0 9± 6 .0 9) % ,脱细胞组为 (6 .71± 2 .1 8) % ,脱细胞加碱处理组为 (1 .6 2± 0 .76 ) % ,组间比较差别均有显著意义 (P<0 .0 1 )。 结论 :MHCObjective:To detect the immunog enicity of the bioscaffold used in building tissue engineered blood vessels.Methods:The carotid arteries of pigs were cut into 2 cm long segme nts and were randomly divided into 3 groups:the control group,the decellulariz ed group,and the decellularized/base treated group.Major histocompatibility c omplex class Ⅰ(MHCⅠ),which was highly expressed in vascular mural cells,was ch osen t o evaluate the antigenic changes of the vessels before and after decellularized treatment.The staining results were analyzed with image analysis system.Results:MHCⅠ was highly expressed in the control group,while after treatments its detectable rate dropped significantly.The ratio of positive gra nular area to the visual area was (24.09±6.09)% in the control group,(6.71±2.1 8 )% in the decellularized group and (1.62±0.76)% in the decellularized/base tre ated group.The differences among groups were significant ( P <0.01).C onclusion:MHCⅠ immunohistochemical staining can serve as a valuable m ethod to detect the antigenicity of the vascular bioscaffold.

关 键 词：组织工程 血管脱细胞支架 免疫组织化学 免疫原性 主要组织相容性复合体I 免疫排斥反应 

分 类 号：R318.12[医药卫生—生物医学工程] R392[医药卫生—基础医学]