大孔树脂柱色谱法制备红花黄色素和羟基红花黄色素A  

Preparation of safflor yellow and hydroxysafflor yellow A by macroporous resin column chromatography

作　　者：金鸣[1] 高子淳[2] 李金荣[1] 臧宝霞[1] 吴伟[1] 

机构地区：[1]北京市心肺血管疾病研究所-首都医科大学附属北京安贞医院药理研究室,北京100029 [2]北京中医药大学中药学院,北京100102

出　　处：《中草药》2004年第1期25-28,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目 (3 0 17114 6)

摘　　要：目的　建立简便有效的制备红花有效部位红花黄色素 (safflor yellow,SY)及有效成分羟基红花黄色素 A(hydroxysafflor yellow A,HSYA)的方法。方法　室温水提减压浓缩所得红花水提液上 D- 4 0 2 0型非极性大孔树脂柱 ,水洗脱糖类等杂质 ,以 30 %和 10 %乙醇洗脱分别得 SY和 HSYA;采用分光光度法和 HPL C法分析水洗脱除糖过程中 SY和 HSYA的损失率及醇洗脱 SY和 HSYA的回收率。结果　聚酰胺薄膜色谱结果显示 SY和 HSYA洗脱液分别可见 4～ 5个和 1个黄色荧光斑点。 HPL C分析结果表明 ,两洗脱液中所含 SY和 HSYA纯度分别为92 .4 %和 83.0 %。在水洗除杂质和稀醇洗脱过程中 ,损失率和回收率分别为 SY:7.5 %和 80 .6 % ,HSYA:1.6 4 %和77.3%。结论　本法适于大规模制备 SY和 HSYA。Object To establish the simple and effective technique for preparing water soluble safflor yellow (SY) and hydroxysafflor yellow A (HSYA) from Carthamus tinctorius L. Methods Aqueous extract prepared through repeated water extraction at room temperature and vacuum evaporation was added to nonpolar macroporous resin column and the impurities such as sugar were washed out by water,then SY and HSYA was eluted by 30% and 10% ethanol individually. The rate of loss and of recovery was determined with spectrophotometry and HPLC analysis. Results It was observed that the polyamide TLC analysis result of SY and HSYA elution was 4—5 or one yellow spot under fluorescent light. It was shown in HPLC analysis that the content of SY and HSYA in two elutions was 92.4% and 83.0% respectively. In the procedure of sugar washing with water or the effective components eluting by diluted ethanol,the rate of loss and recovery are SY: 7.5% and 80.6%,HSYA: 1.64% and 77.3% respectively. Conclusion This technique is suitable to prepare SY and HSYA in a large scale.

关 键 词：红花 红花黄色素 羟基红花黄色素A 大孔树脂柱色谱 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]