吉林双阳梅花鹿核蛋白体蛋白L27 cDNA的克隆  

Cloning of ribosomal protein L27 cDNA from Jilin Shuangyang sika deer

作　　者：魏征人[1] 陈越[1] 杨煜[1] 于永利[1] 

机构地区：[1]吉林大学基础医学院免疫学教研室,吉林长春130021

出　　处：《中草药》2004年第1期75-79,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助 (3 0 70 70 5 )

摘　　要：目的　为了开发我国珍稀动物——吉林双阳梅花鹿的基因资源 ,在分子水平上揭示鹿药材的药理作用。方法　构建了梅花鹿脾脏细胞 c DNA质粒文库 ,克隆了一些看家基因并进行了序列分析。结果　其中的一个序列在核酸及推导的氨基酸序列水平上与编码人、犬、大鼠、小鼠核蛋白体蛋白 L 2 7(ribosom al protein L 2 7) c DNA序列和对应的氨基酸序列高度同源 ,并具有完整的开放阅读框架 (ORF)。结论　发现了双阳梅花鹿核蛋白体蛋白 L2 7全长 c DNA序列 ,此序列已经在 Genbank中登录 ,登录号为 :AF3732 31。Object To develop the gene resources of Jilin Shuangyang sika deer,a rare animal in China and reveal the pharmacological functions of deer medicinal material on the molecular level. Methods A cDNA plasmid library of spleen cells derived from Shuangyang sika deer was set up. Some housekeeping genes were cloned and analyzed. Results One of the cDNA sequences and it's deduced amino acid sequence in deer share high homology with a group of cDNA sequences encoding ribosomal protein L27 in human,rat,Canis and mouse and their corresponding amino acid sequence respectively. It has the completed open reading frame as well. Conclusion A novel full-length cDNA encoding Shuangyang sika deer ribosomal protein L27 is discovered. This sequence has been deposited in Genbank under accession number AF373231.

关 键 词：双阳梅花鹿 核蛋白体蛋白L27 序列分析 

分 类 号：R282.7[医药卫生—中药学]