大鼠移植肠局部CTLA4Ig基因转染的可行性研究  

The Possibility of Local Transfection of CTLA4Ig Gene into Small Bowel Allografts in Rats

作　　者：王一芳[1] 许爱刚[1] 吴文溪[1] 

机构地区：[1]南京医科大学第一附属医院胃肠外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2004年第1期19-21,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省教育厅重点学科基金资助项目(H200212)

摘　　要：目的:探讨CTLA4Ig基因能否在移植小肠局部转染表达。方法:移植前经肠系膜上动脉给供肠灌注脂质体包裹的CT-LA4IgcDNA重组质粒,应用免疫组织学及逆转录多聚酶链反应(RT-PCR)观察移植小肠中CTLA4Ig转基因产物的表达。结果:免疫组织学及RT-PCR结果显示在移植后48h移植小肠中有大量CTLA4Ig转基因产物的表达。结论:经肠系膜上动脉体外灌注脂质体包裹的CTLA4IgcDNA重组质粒可有效转染移植小肠。Objective:To evaluate the possibility of local expression of C TLA4 Ig gene in small bowel allografts. Methods::The donor small bowels were perfus ed ex vivo with the reconstructed plasmid of CTLA4Ig cDNA packaged with lipofect in vector via intra-superior mesenteric artery before transplantation. The CTLA 4Ig transgene expression in the small bowel allografts was assessed by immunohis tology and reverse transcriptase polymerase chain reaction (RT-PCR) after trans plantation. Results:Immunohistology and RT-PCR demonstrated abundant expressio n of CTLA4Ig transgene in the allografts 48 h after transplantation. Conclusion :A single ex vivo intra-superior mesenteric artery infusion of reconstructed p lasmid of CTLA4Ig cDNA packaged with lipofectin can induce efficient transductio n of the small bowel allografts.

关 键 词：小肠移植 CTLA4IG 基因治疗 脂质体 

分 类 号：R656.7[医药卫生—外科学]