固定化啤酒酵母废菌体吸附Pd^(2+)的研究  

STUDIES ON BIOSORPTION OF Pd2+ BY THE IMMOBILIZED SACCHAROMYCES CEREVISIAE WASTE BIOMASS

作　　者：谢丹丹[1] 刘月英[1] 吴成林[1] 傅锦坤[2] 薛茹[2] 古萍英[2] 

机构地区：[1]厦门大学生命科学学院 [2]厦门大学化学化工学院,厦门361005

出　　处：《微生物学通报》2003年第6期29-34,共6页Microbiology China

基　　金：国家自然科学基金(No.29876026)

摘　　要：用2％海藻酸钠与1％明胶混合为包理剂固定啤酒酵母废菌体。SEM、X-射线能谱和TEM研究结果表明,该固定化啤酒酵母废菌体(ISCWB)颗粒中的菌体分布较均匀,ISCWB不仅能吸附Pd^(2+),而且能将Pd^(2+)还原成Pd^0。ISCWB吸附Pd^(2+)的最适pH值为3.5。在30℃～70℃范围内,吸附作用不受温度的影响。吸附作用是一个较快的过程,在最初的5min内吸附量可达最大吸附量的36％。吸附作用受ISCWB浓度、Pd^(2+)起始浓度和共存离子的影响。在起始Pd^(2+)浓度100mg/L、ISCWB浓度1.8g/L、pH3.5和30℃条件下振荡吸附90min,吸附量为40.6mg/g。以0.5mol/L盐酸作为解吸剂解吸率达98.7％。连续吸附与解吸附试验结果表明,ISCWB的最大饱和吸附量为46.3mg/g,解吸率为98％。Saccharomyces cerevisiae waste biomass (SCWB) was entrapped with the mixture of 2% alginate sodium and 1 % gelatin. The results of SEM , X-ray spectrum and TEM analysis showed that the SCWB were uniformly immobilized within the entrapment matrix and Pd2+ could be adsorbed and reduced to Pd0 particles by immobilized SCWB (ISCWB) The optimum pH value of Pd2 + biosorption by ISCWB was 3.5. The temperature in the rage of 30℃-70℃ did not affect the biosorption. The biosorption was a rapid process, reaching 36% of the maximum capacity within the first 5 min. The content of the biomass, Pa2+ initial concentration and the competing metal ions in the solution affected the biosorption. The biosorptive capacity reached 40.6mg/g under the conditions of 100mg Pd2+/L, 1.8gISCWB/L, pH3.5 and 30℃ for 90 min. 98.7% of Pd2+ adsorbed on ISCWB could be desorbed with 0.5mol/L HCl. In packed-bed reactor, the saturation uptake and desorbed efficient were 46.3mg/g and 98% , respectively.

关 键 词：啤酒酵母废菌体 固定化 生物吸附 钯 

分 类 号：Q939.5[生物学—微生物学]