微量液基稀释法测定中药活性成分的体外抗曲霉菌活性  

IN VITRO ANTI-ASPERGILLI ACTIVITY STUDY OF ACTIVE INGREDIENTS OF CHINESE HERBS BY BROTH MICRODILUTION TESTING

作　　者：谢小梅[1] 许杨[2] 付颖媛[3] 

机构地区：[1]江西中医学院基础部,南昌330006 [2]江西-OAI联合研究院南昌大学食品科学教育部重点实验室,南昌330047 [3]江西医学院,南昌330006

出　　处：《微生物学通报》2003年第6期89-92,共4页Microbiology China

基　　金：国家自然科学基金(No.30160099)

摘　　要：通过测定中药活性成分肉桂醛和柠檬醛对常见深部条件致病性真菌黄曲霉、烟曲霉的抗菌活性,为建立中药抗曲霉菌药敏试验标准提供参考依据。参照美国国家临床试验标准化委员会(National Committee for Clinical Laboratory Standards,NCCLS)提出的标准,用微量液基稀释法分别测定肉桂醛和柠檬醛对黄曲霉、烟曲霉的抗菌活性。肉桂醛对黄曲霉、烟曲霉最低抑菌浓度(Minimal inhibitory concentration,MIC)分别为:0.100μg/mL、0.050μg/mL,柠檬醛对黄曲霉、烟曲霉的MIC分别为:2.600μg/mL、0.650μg/mL。中药活性成分肉桂醛和柠檬醛具有高效抗曲霉作用。该研究可为制定中药抗曲霉作用评价标准提供参考依据。To develop a method to estimate the anti-Aspergilli activity of active ingredients of Chinese herbs. With the broth microdilution testing procedure proposed by NCCLS, anti-Aspergilli activity of active ingredients ( cinnamalde-hyde, cinn. and citral) of Chinese herbs was determined.The MIC values of cinn. to Aspergillus flavus, A.fumigatus were 0.100μg /mL , 0.050μg/mL.The MIC values of citral to A. flaws, A.fumigatus were 2.600μg/mL, 0.650μg/ mL.These results demonstrate that citral and cinn. have high potency activity against Aspergillus spp. .The research may provide references to establishing a standard for evaluating the effect of anti-Aspergilli Chinese herbs.

关 键 词：抗曲霉活性 微量液基稀释法 中药活性成分 

分 类 号：R285[医药卫生—中药学]