胰岛素对PC12细胞神经型一氧化氮合酶表达及活性的影响  

Insulin Regulated The Expression and Activity of Neuronal Nitric Oxide Synthase in PC12 Cells

作　　者：袁中瑞[1] 鲁丹丹[1] 董晓敏[2] 古力努尔[1] 程晟[1] 卢景雰[1] 

机构地区：[1]北京大学医学部天然药物及仿生药物国家重点实验室 [2]北京大学医学部细胞生物学教研室,北京100083

出　　处：《生物化学与生物物理进展》2003年第6期960-964,共5页Progress In Biochemistry and Biophysics

摘　　要：为探讨胰岛素对神经细胞中神经型一氧化氮合酶 (nNOS)的表达及活性的影响 ,应用流式细胞术、原位杂交、电子自旋共振等技术方法研究胰岛素对PC12细胞中神经型一氧化氮合酶的影响 .胰岛素作用PC12细胞9h后 ,神经型一氧化氮合酶的免疫荧光强度显著升高 ,且呈浓度依赖关系 ,其最大效应为对照的 (155± 13 ) %(P <0 0 1,n =3 ,t test) .加入胰岛素 (16mU /L ,6h)也能够显著上调nNOSmRNA的表达 ,为对照的 (182± 13 ) % (P <0 0 1,n =3 ,t test) .另外加入胰岛素 (16mU /L)作用 9h后 ,神经型一氧化氮合酶的活性也显著升高 ,为对照的 (167± 15) % (P <0 0 1,n =4,t test) .由上述结果可知 。To research the effect of insulin on the expression and activity of the neuronal nitric oxide synthase in neural cells, the effects of insulin on the expression and activity of neuronal nitric oxide synthase were investigated in PC 12 cells, by using flow cytometry, in situ hybridization and electron spin resonance (ESR) techniques. After incubation with insulin for 9 h, the expression of nNOS protein was significantly increased by insulin in a concentration-dependent manner, with a maximal increase of about 55% of the control values (P < 0.01, n = 3, t-test). The transcription of nNOS was also significantly increased by insulin (16 mU/L, 6 h), reaching (182 &PLUSMN; 13)% of the control values (P <0.01, n = 3, t-test). In addition, significant increase of the activity of nNOS in PC12 cells was also detected after incubation with insulin (16 mU/L) for 9 h, with an increase of 67% of the control values (P < 0.01, n = 4, t-test). It can be concluded that insulin can upregulate the expression and activity of neuronal nitric oxide synthase in PC12 cells.

关 键 词：胰岛素 神经型一氧化氮合酶 流式细胞术 原位杂交 电子自旋共振 PC12细胞 中枢神经系统 

分 类 号：Q42[生物学—神经生物学] Q26[生物学—生理学]