哺乳动物克隆的研究进展  

Progress in mammalian clone

作　　者：王荣[1] 李文雍[1] 

机构地区：[1]阜阳师范学院生物系,安徽阜阳236032

出　　处：《阜阳师范学院学报（自然科学版）》2003年第4期21-24,共4页Journal of Fuyang Normal University(Natural Science)

摘　　要：哺乳动物体细胞克隆是20世纪末生命科学领域最引人注目的高新技术,该技术在农业、生物学和医药等方面具有广泛的应用前景。近年来克隆技术发展迅速,多种哺乳动物相继克隆成功,但也存在着克隆效率太低、克隆动物表型正常而实质异常等问题。本文在近年来克隆技术研究的基础上,对克隆的概念、研究历史以及克隆的方法、过程进行了综述,展望了克隆的意义及克隆动物的应用前景,分析了目前克隆技术尚存在的问题,并提出了解决这些问题的关键。Mammalian cloning is the most remarkable high technique in the end of 20th century in life science field. It is very promising in agriculture, biology and pharmacology. Cloning technique has developed rapidly so far, Although many mamma lian species have been cloned from adult somatic cells, the efficiency is still very low, the offspring are aberrant with nounal surface, and so on. This reriew gives the definition of mannalian done, It reviews the research histoly of cloning, and deals with the methods and process based on prevenient studies. Ths inearring and applicatiow have been prospected, and some problems existing in mammalian clene have been analoped.

关 键 词：哺乳动物 体细胞克隆 显微操作 去核 融合 重构胚 

分 类 号：Q959.8[生物学—动物学] Q785