Preparation, characterizationand preliminary in vivo stud-ies of inactivated SARS-CoV vaccine  

作　　者：TANGLin WANGJian QINEde ZHUQingyu YUMan DINGZhifen SHIHuiying CHENGXiaojie WANGCaiping CHANGGuohui LIShuangli ZHANGXumin CHENXishu YUJun CHENZe 

机构地区：[1]BeijingGenomicsInstitute,ChineseAcademyofSciences,Beijing101300,China [2]InstituteofMicrobiologyandEpidemiology,AcademyofMilitaryMedicine,Beijing100071,China [3]NationalVaccineandSerumInstitute,Beijing100024,China [4]WuhanInstituteofVirology,ChineseAcademyofSciences,Wuhan430071,China

出　　处：《Chinese Science Bulletin》2003年第23期2621-2625,共5页

摘　　要：A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and elec-tron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.A large quantity of SARS-CoV virus was pro-liferated in Vero cells, inactivated with b-propiolactone, then purified by Sepharose 4FF column chromatography to pre-pare inactivated vaccine. The vaccine was identified by Western blot, mass spectrographic analysis, ELISA and elec-tron microscopy. The vaccine with or without aluminum hydroxide adjuvant was inoculated into female BALB/c mice at different dosages. The result showed that the antibodies to SARS-CoV were induced in the mice. The antibody levels induced by the vaccine with aluminum hydroxide were higher than those without aluminum hydroxide.

关 键 词：疫苗 非典型肺炎 SARS β-丙内酯 中国 

分 类 号：R563.1[医药卫生—呼吸系统]