新疆杨高效遗传转化系统的建立  

Establishment of system with high frequency for genetic transformation of Populus alba var. pyramidalis

作　　者：诸葛强[1] 王婕琛[1] 陈英[1] 黄敏仁[1] 王明庥[1] 

机构地区：[1]南京林业大学林木遗传和基因工程重点实验室,江苏南京210037

出　　处：《植物资源与环境学报》2003年第4期6-10,共5页Journal of Plant Resources and Environment

基　　金：国家研究与开发专项(JY03 B 25);江苏省高新技术资助项目(BG2001313)

摘　　要：选择新疆杨(PopulusalbaL.var.pyramidalisBge.)为遗传转化受体材料,为建立根癌农杆菌介导新疆杨高效遗传转化系统,从预培养时间、侵染时间、共培养时间、添加乙酰丁香酮(AS)的时机、共培养培养基中添加乙酰丁香酮浓度、侵染菌液的制备方法、外植体继代方式等7个方面优化筛选。结果显示较合适的转化系统为:预培养8h,农杆菌菌液(OD600=0.4)侵染15min,共培养5d,侵染菌液的最优制备方法是液体培养活化农杆菌2次加离心收集菌体重悬,共培养培养基中添加乙酰丁香酮80μmol/L。新疆杨叶盘转化频率可达38.10%。Several factors affecting transformation of \%Populus alba \%L. var. \%pyramidalis \%Bge. were studied by infecting with \%Agrobacterium tumefaciens \%LBA4404, such as pre\|culture time, infection time, co\|culture time, acetosyringone (AS) appending method, AS concentration in the co\|culture medium, bacterium liquid preparation method, explants state. An effective protocol in optimized conditions which were 8 h for pre\|culture, 15 min for infection, 5 d for co\|culture and dosage 80 μmol/L AS in co\|culture medium for transformation of leaf discs of \%P. alba \%var. \%pyramidalis \%was developed and the frequency of transformation reached to 38.10%.

关 键 词：新疆杨 遗传转化系统 根癌农杆菌 农杆菌 

分 类 号：S792.11[农业科学—林木遗传育种] Q943.2[农业科学—林学]