前表面三维荧光技术检测鼠尾胶原蛋白中老年色素类物质随龄的变化  

Age-dependent alteration of rat-tail pigments determined by a front-face 3D-fluorescent technique

作　　者：廖艳阳[1] 谢渝湘[1] 李国林[1] 印大中[1] 

机构地区：[1]湖南师范大学生命科学学院,湖南长沙410081

出　　处：《中国老年学杂志》2003年第12期845-847,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助 (30 0 70 874);湖南教育部基金资助(531 1 2 - 0 82 0 )

摘　　要：目的　观察老年色素类物质在生物体内含量的随增龄变化。方法　用前表面三维荧光技术和荧光分光光度法检测老年鼠和幼年鼠的鼠尾胶蛋白、水相和有机抽提液相及抽提后残渣物质中荧光物质的变化。结果　鼠尾胶原蛋白、水相和有机抽提液相中荧光物质的随龄增长均比较明显 ;同时 ,抽提后残渣直接检测也观察到了这种随龄的变化。本实验中 ,组织中没有检测到老年色素荧光物质的变化 ,激发波长与发射波长均没有发生明显的红移。结论　老年色素类物质在生物体内的含量随时间推移而积累。前表面三维荧光技术在对老年色素的研究当中将成为一个非常有用的工具。Objective To observe the age-dependent changes of autofluorescence in rat-tail collagen.Methods Rat-tail collagen, water and chloroform phases and the residue part were measured by three dimensional (3D) front-face fluorometry and normal spectrofluorometer respectively.Results The age-related increase of fluorescent emission in rat-tail collagen, water and organic extractions was significant, so was in the residue after extraction. Fluorescent red-shift was not discernible in an age dependent manner in this system.Conclusions Age pigments have been found to be accumulated with aging in biology. The front-face fluorescence measurement was proved to be a useful tool in studying pigment alterations related to aging process.

关 键 词：胶原蛋白 老年色素 前表面三维荧光技术 衰老 动物实验 

分 类 号：Q591.5[生物学—生物化学]