麦胚提取物对去卵巢大鼠无机盐和骨形成蛋白2的影响  

Effects of wheat embryo extract on mineral and bone BMP_2 in ovariectomized rats

作　　者：田玉慧[1] 李万里[2] 董艳臣[3] 李永浦[4] 

机构地区：[1]新乡医学院预防医学教研室,河南新乡453003 [2]新乡医学院微生物免疫学教研室 [3]新乡医学院机能实验室 [4]新乡医学院

出　　处：《中国老年学杂志》2003年第12期863-865,共3页Chinese Journal of Gerontology

基　　金：河南省科技厅 (0 1 2 4 1 70 736)

摘　　要：目的　探讨麦胚提取物对去卵巢引起骨质疏松大鼠无机盐和骨形成蛋白 2 (BMP2 )的影响。方法　 2 1只雌性 SD大鼠随机分为假手术组、去卵巢模型组、去卵巢加麦胚提取物组 (麦胚提取物 1 0 0 mg/ kg· d) ,1 0 w后测定血清无机盐钙、磷、钠、钾、AL P含量 ,用 SP免疫组织化学检测骨 BMP2 表达。结果　去卵巢组大鼠试验 1 0 w后血清和尿钙、钠和血清 ALP含量显著升高 ;而血清和尿钾降低 ,骨 BMP2 表达下降 (P<0 .0 5或 P<0 .0 1 )。去卵巢加麦胚后大鼠的尿钙、钠和血清 ALP含量显著降低 ,骨 BMP2 表达增高 (P<0 .0 5)。结论　麦胚提取物可调节去卵巢大鼠血清和尿中钙、钠、钾 ,促进 BMP2 表达 ,预防去卵巢大鼠骨质疏松。Objective To study the effects of wheat embryo extract on mineral, ALP and bone BMP 2 in osteoporosis rats induced by ovariectomizing.Methods Twenty one female SD rats were randomly divided into three groups: sham-operated group, ovariectomized model group, ovariectomized added wheat embryo group (100 mg·kg -1 ·d -1 ).The calcium, phosphorus, sodium, potassium and ALP content of serum and urine was determined after ten weeks. The Streptavidin-Peroxidase Histostain TM -Plus Kit was used to detect bone BMP 2 expression.Results The calcium, sodium and ALP content of serum and urine increased significantly in ovariectomized group, but potassium content of serum and the expression of bone BMP 2 decreased(P<0.05 or P<0.01).The content of calcium, sodium and ALP in urine significantly decreased and the expression of bone BMP 2 increased in ovariectomized added wheat embryo group compared with ovariectomized group.Conclusions The wheat embryo extract might regulate the calcium, sodium, potassium in serum and urine and promote the expression of bone BMP 2 and the prevent osteoporosis of ovariectomized rats .

关 键 词：麦胚提取物 去卵巢大鼠 无机盐 骨形成蛋白2 骨质疏松 成骨细胞 

分 类 号：R965[医药卫生—药理学] R681[医药卫生—药学]