同期输注供者地塞米松诱导脾细胞对肝移植大鼠免疫影响  

Immunological effects of simultaneous injection of dexamethasone-induced apoptotic donor spleen cells in rats with liver transplantation

作　　者：刘静[1] 高毅[1] 孙尔维[1] 张志[1] 汪爽[1] 钟世镇[2] 

机构地区：[1]第一军医大学珠江医院器官移植科,广东广州510282 [2]第一军医大学临床解剖学研究所,广东广州510515

出　　处：《第一军医大学学报》2003年第12期1249-1252,共4页Journal of First Military Medical University

基　　金：国家自然科学基金(39970705)~~

摘　　要：目的探讨肝移植同时输注地塞米松体内诱导凋亡的供体脾细胞对受体肝移植免疫的影响。方法实验分为对照组、单纯供体地塞米松处理组,单纯输供者脾细胞组和地塞米松诱导供者凋亡脾细胞输注组。每组Wistar和SD大鼠各10只。用地塞米松3 mg·d-1·kg-1·b.w.处理Wistar大鼠3 d后,第4天行大鼠肝移植。观察术后第7天肝功能(ALT、TBil)的变化、病理改变和受体生存期。结果肝移植同时输注凋亡细胞组和单纯输注脾细胞组的谷氨酸转移酶(ALT)、血清总胆红素(TBil)较对照组和单纯地塞米松处理组显著升高(P<0.05);生存期明显缩短(P<0.05),病理改变呈急性重度排除反应,而对照组仅呈急性轻度排除反应。结论肝移植同时输注地塞米松体内诱导供体凋亡的脾细胞促进受体对移植肝的排除反应,凋亡细胞未被及时吞噬可能是引起排斥反应的重要原因。Objective To study the immunological effects of simultaneous injection of apoptotic donor spleen cells induced bydexamethasone in rats with liver allotransplantation. Methods Four groups of rats were used in this study, each consisting of 10 SD rats and 10 Wistar rats with the former as the recipients and the latter as the donors for liver transplantation. In one ofthe groups, the recipient rats also received infusion of apoptotic spleen cells (5×107) of the donors induced by an intraperitonealinjection of dexamethasone (at a daily dose of 3 mg/kg) for 3 days before liver transplantation, while in another, the recipientreceived untreated donor spleen cells. In the third group, the donor was treated with dexamethasone leaving the last groupserving as the control group. The blood alanine transaminase (ALT), total bilirubin (TBil), pathological changes of the graftand survival time of the recipients were observed. Results The recipients with apoptotic donor spleen cell infusion had muchhigher ALT and TBil levels than those of the other 3 groups (P<0.05), exhibiting significantly shortened survival time andseverer acute allograft rejection, as compared with the mild acute rejection in the control group (P<0.01). Conclusion Simultaneous injection of apoptotic donor spleen cells induced by dexamethasone in rats with liver transplantation aggravatesacute allograft rejection, one of the possible mechanisms of which may lie in the failure of timely removal of the apoptoticcells that release inflammatory factors.

关 键 词：同期输注 供者 地塞米松 脾细胞 肝移植 大鼠 免疫学 

分 类 号：R657.3[医药卫生—外科学] R977.11[医药卫生—临床医学]