虎杖甙对脂多糖刺激下中性粒细胞趋化反应的影响  

Effect of polydatin on lipopolysaccharide-induced leucocyte chemotaxis

作　　者：黄海潇[1] 赵清[1] 金春华[1] 

机构地区：[1]第一军医大学休克微循环重点实验室,广东广州510515

出　　处：《第一军医大学学报》2003年第12期1253-1256,共4页Journal of First Military Medical University

基　　金：国家自然科学基金(9870936)~~

摘　　要：目的从白细胞趋化性变化中探讨虎杖甙治疗感染性休克的作用机制.方法以趋化寡肽fMLP为趋化因子,用趋化小室观察了正常时中性粒细胞的趋化活性以及脂多糖(LPS)、虎杖甙(PD)和PD对LPS刺激后中性粒细胞的趋化指数的改变.结果正常情况下,中性粒细胞表现出一定的趋化活性(趋化指数为4.96±±0.69).在10、100、1000ng/ml LPS刺激后,中性粒细胞趋化性较正常时显著增强(白细胞趋化指数各为8.94±1.73,10.31±1.180和7.12±1.46,LPS刺激组显著高于正常对照组)(P＜0.05).PD在0.8～0.008 mg/ml范围内均能抑制LPS的作用,使增高的白细胞趋化细胞数减少,以PD浓度0.08 mg/ml时效果最显著,其趋化指数可达1.95±0.17.PD作用5～60 min,均能导致白细胞的趋化细胞数下降.当LPS浓度在10～1 000 ng/ml时,各PD治疗组与正常对照组比较均有显著性差异(P＜0.05).但不同浓度PD对正常中性粒细胞趋化性没有明显作用(P＞0.05).结论PD可调节LPS性炎症反应条件下白细胞的趋化性,可能对防治炎症和感染性休克有重要作用.Objective To understand the action mechanisms of polydatin (PD) in the treatment of septic shock in view of itseffect on lipopolysaccharide (LPS)-induced chemotaxis of the leucocytes. Method Chemotactic chamber assay was used toinvestigate the regulative role of PD in chemotaxis of the neutrophils in response to LPS stimulation. Results The chemotacticindex of normal neutrophils was 4.96±0.69, which was significantly increased by LPS stimulation. LPS stimulation at thedoses of 10, 100, and 1000 ng/ml resulted in the elevation of the chemotactic index of the neutrophils to 8.94±1.73, 10.31±1.180 and 7.12±1.46 respectively (P<0.05), an effect potently reversed by the application of PD at the concentrations rangingfrom 0.008to 0.8mg/ml,ofwhich 0.08mg/mlwasthemosteffectiveconcentrationthatproducedadecreaseofthechemotactic index to 1.95±0.17. In addition, PD exhibited obvious anti-LPS effect after treatment for 5 to 60 min (P<0.05),while showing no influence on the chemotaxis of normal neutrophils (P>0.05). Conclusion PDcanregulateneutrophilchemotaxis in inflammatory reactions and may play a crucial role in the treatment of infections and inflammation.

关 键 词：虎杖甙 脂多糖 中性粒细胞 细胞趋化反应 治疗 感染性休克 

分 类 号：R285[医药卫生—中药学]