小鼠胚胎发育中期心脏细胞增殖与凋亡的研究  

Cell Proliferation Apoptosis and the Expression of Related Genes in the Developing Heart of Mouse

作　　者：梁亦龙[1] 谢永芳[1] 聂永萍[2] 舒坤贤[1] 

机构地区：[1]重庆邮电学院生物信息学院,重庆400065 [2]重庆邮电学院计算机学院,重庆400065

出　　处：《海南大学学报（自然科学版）》2003年第4期344-347,共4页Natural Science Journal of Hainan University

基　　金：国家自然科学基金资助项目(30000084);重庆邮电学院青年科技基金资助项目(2001-18A)

摘　　要：采用免疫组织化学及切口末端标记法(TUNEL),对胚胎中期的小鼠心脏组织进行检测,结果表明:小鼠心脏发育过程中的细胞增殖与凋亡相伴存在,与心脏发育变化密切相关.而用原位杂交的方法检测,P21、Ki ras基因在胚胎心脏发育中期并没有表达,说明这2个细胞周期调控基因在胚胎心脏发育中期并不起作用,可能与其他的基因有关.Immunohistochemistry and TUNEL techniques were adopted to observe the variation of cell proliferation and apoptosis and the expression characteristics of P 21 and Ki-ras. We discovered that cell proliferation was accompanied by apoptosis during the development of mouse heart. The dynamic changes of both ratios might be correlated to the different structures appearing in different developmental process. The possible function of P 21 and Ki-ras might be not correlated to the developmental process of middle embryo heart.

关 键 词：小鼠 胚胎发育 心脏 细胞增殖 细胞凋亡 P21基因 KI-RAS基因 

分 类 号：Q132.4[生物学—普通生物学] Q253