硫辛酸对海马CA_1区锥体细胞凋亡的抑制作用  

Inhibitory Effect of α-Lipoic Acid in the Apoptosis of Pyramidal Cells in CA_1 Region of the Hippocampus

作　　者：刘学忠[1] 崔旭[2] 卞建春 任建新[1] 刘宗平[1] 王捍东[1] 王宗元[1] 

机构地区：[1]扬州大学畜牧兽医学院动物医学系,江苏扬州225009 [2]解放军总医院老年医学研究所,北京100853

出　　处：《动物医学进展》2004年第1期85-87,共3页Progress In Veterinary Medicine

基　　金：国家重点基础研究发展规划项目 (G2 0 0 0 0 5 70 0 0 ) ;江苏省自然科学基金项目 (BK19990 93) ;扬州大学畜牧兽医学院科研基金项目 (2 0 0 30 0 6 )

摘　　要：采用股动脉放血并双侧颈总动脉夹闭制作全脑缺血再灌注模型 ,于术后 1 d处死动物 ,取脑 ,制作石蜡切片 ,通过 HE染色、Tunel检测 ,以观察海马 CA1区锥体细胞的损伤形式 ,并探讨α-硫辛酸 (α-lipoic acid,L A)对实验性脑缺血再灌注的神经保护作用。结果表明 :在短暂性全脑缺血中 ,海马锥体细胞存在着凋亡和坏死两种死亡形式 ;与对照组相比 ,缺血再灌注后1 d,海马 CA1区损伤较重 ,出现了较多的凋亡细胞 ,而 L A可明显抑制锥体细胞的凋亡。实验证明 :在脑缺血再灌注损伤中 ,细胞凋亡是神经元死亡的一种重要形式 ;LBilateral occlusion model of rats was made and killed on 1d after reperfusion following 10 min forebrain ischemia,then paraffin sections were made to observe the changes of pyramidal cells in CA1 region of the hippocampus and study neuroprotective effect of α lipoic acid(LA)in cerebral ischemia reperfusion(IR) injury through HE and Tunel staining,the results showed:apoptosis and necrosis are two patterns of cell death in the course of rat transient global ischemia;compared with that in the control group,the injury of CA 1 region in the hippocampus was severe, lots of apoptotic cell was seen and LA can inhibit the apoptosis of pyramidal cells.It has been tested that apoptosis may be an important form in the course of cerebral ischemia reperfusion(IR) injury and LA can protect brain tissue from IR injury.

关 键 词：硫辛酸 海马 CA1区 锥体细胞 细胞凋亡 抑制作用 

分 类 号：S968.8[农业科学—水产养殖]