铅性肾病动物模型的建立  

Establishment of a Model of Lead-Induced Nephropaphy

作　　者：黄瑞雪[1] 

机构地区：[1]中南大学湘雅医学院公共卫生学院劳卫教研室,湖南长沙410078

出　　处：《动物医学进展》2004年第1期88-90,共3页Progress In Veterinary Medicine

基　　金：湖南省卫生厅 (980 8)

摘　　要：为了建立铅性肾损害动物模型 ,按随机原则将 3 6只 SD大鼠分为对照组、模型组。对照组用蒸馏水灌胃 ,1 h后 ,腹腔注射无菌生理盐水 ;染毒组动物用 0 .5%醋酸铅 5mg/ kg体重腹腔注射 ,每周五次 ,连续 1 2周。结果 :模型组自实验第 3周开始肾铅、尿蛋白、尿 r-GT活性和尿 δAL A等均极显著地高于对照组 (P <0 .0 1 )。肾近曲小管上皮细胞空泡变性及细胞内线粒体出现丢失。结论 :大鼠腹腔注射 0 .5%醋酸铅 (5mg/ kg体重 )In order to build a model of lead induced nephrotoxicity,a subchronic study was performed in rats.SD rats were randomly assigned to two groups: control group,lead treated group.Rats of control group were given deionized water by gavage one hour prior to being injected with 0.9%/saline solution by i.p. The lead treated group were injected with 0.5%/lead acetate(5 mg/kg body weight)by i.p. All of these treatments were done once a day, 5 days/week for 12 weeks.There was significant increase of renal lead,urinary protein,urinary r GT and urinaryδ ALA in lead treated group compared to control group(P<0.01).Light microcopy of kidney revealed morphological changes of the group mainly in the epithelial cells of proximal tubules.Conclusion: 0.5% lead acetate(5 mg/kg body weight),with which rats were injected,can cause lead induced nephropathy.

关 键 词：铅性肾病 动物模型 模型建立 醋酸铅 

分 类 号：S856.9[农业科学—临床兽医学]