骨髓源间充质干细胞在异基因小鼠免疫器官内的分布及其免疫功能调节作用  

The distribution of bone marrow-derived mesenchymal stem cells in immune organ of allogenic mice and the immunoregulatory effects

作　　者：邓为民[1] 韩钦[1] 葛薇[1] 尤胜国[1] 李长虹[1] 张伟[1] 邓鸿业[1,2] 赵春华[1] 

机构地区：[1]中国医学科学院中国协和医科大学血液学研究所,血液病医院实验血液学国家重点实验室,天津300020 [2]北京大学医学部,北京100083

出　　处：《中国免疫学杂志》2004年第1期40-45,共6页Chinese Journal of Immunology

基　　金：863计划(2002AA205061);CMB(01-748);北京市科委基金(2002-489)资助

摘　　要：目的:探讨骨髓源间充质干细胞(Bone MARROW MESENCHYMAL STEM CELLS,BMSC)在异基因小鼠免疫器官内的分布及其免疫调节作用。方法:以CM-DiI荧光染料示踪BMSC的体内分布情况,并辅以PCR检测Y染色体的方法进一步鉴定;体外实验采用MTT法、ELISA和FACS等方法检测BMSC的免疫调节作用。结果:BMSC可进入并较长期(30天)存在于异基因小鼠免疫器官内;在体外,BALB/C小鼠的BMSC对由ConA诱导的BALB/C和C57BL/6(B6)和BXSB小鼠的T细胞增殖均有抑制作用;而对前两种小鼠由LPS诱导的B细胞增殖和分泌Ig方面表现为促进作用,对BXSB小鼠由LPS诱导的B细胞增殖和Ig分泌有抑制作用。BALB/C小鼠的BMSC对BALB/C和B6小鼠由ConA诱导的IL-4生成细胞的数量无明显影响,却可降低由Co-nA诱导的两种品系小鼠的IFN-γ生成细胞的数量;但对于BXSB小鼠却不同,BALB/C的BMSC可降低由ConA诱导的BXSB小鼠的IL-4生成细胞的数量,而提高由ConA诱导的IFN-γ生成细胞的数量。结论:异基因BMSC不但可进入受体的免疫器官,且可较长期(30天)存在;另外,BMSC对同基因正常、异基因正常和异基因自身免疫病的个体均有一定程度的免疫调节作用。Objective:To investigate the distribution of the murine bone marrow-derived mesenchymal stem cells in immunoorgans of allogeniec mice and their regulatory effects.Methods: In this work, used a available fluorescence dye, CM-DiI, as a tracing label, and Y-chromosome-specific PCR to detect the distribution of BMSC in vivo. And used MTT assay, ELBA, FACS to value the effects of BMSC on the murine immunocompetent cells. Results: BMSCs not only can enter the immunoorgans of allogeneic mouse, but also can exist there for at last 30 days. Ex vivo tests show:(1)BALB/C BMSCs can suppress the T cells proliferation of BALB/C, B6 and BXSB mice stimulated by ConA.(2) BALB/C BMSCs can promote the B cells proliferation and Ig secretion of both BALB/C and B6 mice stimulated by LPS. But they decreased the B cells proliferation and Ig secretion of BXSB mice. (3)BALB/C BMSCs can reduce the IFNγ-producing cell count of BALB/C and B6 mice stimulated by ConA. But showed no affect on their IL-4-producing cell count. For BXSB mice, BALB/C BMSC can cut down the IL-4-produc-ing cell count, but can augment the IFNγ-producing cell count. And all above effects appear as a dose-dependant fashion. Conclusion: Allogeneic BMSC can enter the immunoorgans of recipient, and can exist there for relatively long time (at last 30 days). In addition, BMSC shows some immunoregulatory effects on normal syngeneic, normal allogeneic and allogeneic mice with autoimmune disease.

关 键 词：骨髓源间充质干细胞 免疫器官 分布 免疫活性细胞 免疫调节 

分 类 号：R392.3[医药卫生—免疫学]