“四物汤”中当归有效部位及有效成分的研究  

Analysis of the Effective Part and Effective Component of Angelica Sinensis in Siwutang

作　　者：梁明金[1] 贺浪冲[1] 

机构地区：[1]西安交通大学药学院,西安710061

出　　处：《分析化学》2004年第1期83-86,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .2 0 0 75 0 2 0 )

摘　　要：研究发现“四物汤”中的当归对家兔主动脉血管有舒张作用的有效部位及有效成分。采用超临界CO2 流体萃取当归中的脂溶性部位 ,经柱层析法分离和高效液相色谱法纯化 ,并结合细胞膜色谱法筛选和气相 质谱联用色谱法鉴定 ,确定当归中的有效成分。结果表明 :当归脂溶性部位经柱层析分离得到的正己烷 醋酸乙酯洗脱部位为有效部位 ,其中藁本内酯。To find the effective part and effective components of Angelica sinensis in Siwutang which can produce an arteriolar vasodilation in rabbit. In this paper, the lipophilic extract of Angelica sinensis was obtained by supercritical fluid CO 2 extraction. The effective part and the effective component in it were separated and identified by using systematic chromatographic methods including column liquid chromatography (CLC), high performance liquid chromatography, cell membrane chromatography and gas chromatography-mass spectrometry. The results showed that the effective part was the elute of hexane-ethyl acetate from the extract separated by the silica CLC, and the effective components in the part were ligustilide, dimethyl phthalate and diethyl phthalate respectively.

关 键 词：“四物汤” 当归 有效成份 气相-质谱联用色谱法 药理作用 

分 类 号：O657.63[理学—分析化学]