早期营养干预改善IUGR大鼠胰岛素抵抗及其与血清瘦素的关系  

Early Nutrition Intervention Improve Insulin Resistance in Rats Born with IUGR and Their Relation with Serum Leptin

作　　者：丘小汕[1] 沈振宇[1] 黄婷婷[1] 杜敏联[1] 柯志勇[1] 赖峰[1] 

机构地区：[1]中山大学附属第一医院儿科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2003年第6期568-572,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科委重点攻关基金资助项目(99M04815G)

摘　　要：【目的】探讨生后早期不同饮食构成喂养对宫内生长迟缓(IUGR)大鼠胰岛素抵抗的远期影响及其与瘦素、腹部内脏脂肪的相关关系。【方法】IUGR新生雌鼠48只和正常新生雌鼠10只随机分为5组予下述相应饮食饲料喂养母鼠3周:①IUGR模型组(S/N组)予常规饮食,②IUGR高碳水化合物饮食组(A组),③IUGR高脂肪饮食组(B组),④IUGR高蛋白质饮食组(C组)⑤正常对照组(C/N组)予常规饮食。第4周起各组幼鼠断乳后均予常规饮食饲料喂养至实验结束。各组大鼠于12周(成年期)分别测定体质量、肾周脂肪质量(肾脂)、血清瘦素、血糖、胰岛素并计算胰岛素敏感指数(ISI)、胰岛素抵抗指数(IRI)。【结果】12周时IUGR模型组大鼠肾脂增多,血清瘦素和IRI升高、ISI下降(P<0.05)。IUGR高蛋白饮食组体质量(242.6±17.5)g虽高于C/N组(192.1±37.2)g,但与S/N组(213.4±27.3)g比较无显著性差异(P>0.05),且不伴肾脂(1.46±0.67)g增多,血清瘦素(0.43±0.26)μg/L、ISI(4.47±0.45)和IRI(0.78±0.45)也与正常对照组(1.41±0.42)g,(0.42±0.34)μg/L,4.46±0.42和0.77±0.31比较差异无统计学意义(P>0.05)。【结论】生后哺乳期给予高蛋白质饮食早期营养干预然后恢复正常饮食,可使IUGR大鼠既能达到体格追赶生长。To study the long term effects of different diets with the same caloric on body weight, abdominal fat, serum leptin level, insulin sensitive index(ISI), and insulin resistant index(IRI) in rats born with IUGR. IUGR models were built by maternal nutrition restriction. 48 newborn IUGR female pups and 10 normal female pups were randomly divided into 5 groups, the mother rats in each group were given the following diet from the one to three week after birthing respectively: ①IUGR control group (S/N group)fed with normal diet.②IUGR high carbohydrate diet group(A group). ③IUGR high fatty diet group(B group).④IUGR high protein diet group (C group).⑤The normal control group(C/N group) were fed with normal diet . All pups in each groups were fed with normal diet from the 4th week to the end of the experiment after ablactation. At the 12th week of life(adulthood), the body weight, perirenal fat weight , serum leptin level, blood glucose, insulin concentration were measured, and the ISI and IRI were calculated. At the 12th week of life, there were higher perirenal fat weight in the IUGR control group, and serum leptin concentration and IRI were higher. ISI was lower than that in the normal control group( P< 0.05). The body weight(242.6±17 5)g of IUGR high protein diet group was higher than that of the normal control group(192.1±37.2)g, but no significant difference compared with IUGR control group(213.4±27.3)g, P >0.05. Perirenal fat(1.46±0.67) g, serum leptin concentration(0.43±0.26)μg/L, ISI(4.47±0.45) and IRI(0.78±0.45)were no significant differences between the IUGR high protein diet group and the normal control group[1.41±0.42)g,(0.42±0.34)μg/L,4.46±0.42 and 0.77±0.31], P >0.05. [Conclusion]By the effect of “nutritional programming”, the high protein diet during lactation is the reasonable approach of early nutritional intervention, which not only meet the catch up growth but also avoid insulin resistance in rats born with IUGR.

关 键 词：早期 营养干预 IUGR大鼠 胰岛素抵抗 血清瘦素 宫内生长迟缓 

分 类 号：R714.43[医药卫生—妇产科学]