Rapid Constructing a Genetic Linkage Map by AFLP Technique and Mapping a New Gene tms5  

用AFLP标记快速构建遗传连锁图谱并定位一个新基因tms5(英文)

作　　者：贾建航[1] 李传友[1] 邓启云[1] 王斌[1] 

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《Acta Botanica Sinica》2003年第5期614-620,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家转基因植物研究与产业化专项 (J99_A_0 0 2 ) ;国家重点基础研究发展规划项目 (2 0 0 1CB10 880 2 )。~~

摘　　要：In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.报导了一个分子标记连锁图的快速构建方法。通过对水稻 (OryzasativaL .)“安农S_1”和“南京 11”的F2 分离群体的AFLP分析找到了 14 2个AFLP标记 ,用这 14 2个AFLP标记以及已定位的 2 5个SSR标记和 5个RFLP标记构建了水稻 12个染色体的分子标记连锁图 ,该图覆盖水稻基因组的 15 37.4cM ,相邻标记间的平均间距为 9.0cM ,这是在国内建立的第一张AFLP标记连锁图。在建立连锁图谱的同时把一个新基因tms5 (水稻温敏核不育基因 )定位在第 2染色体上。

关 键 词：AFLP rapid constructing genetic linkage map rice thermosensitive genic male sterile gene tms5 

分 类 号：Q943[生物学—植物学]