钯卟啉探针与核酸相互作用的研究  

A study on the interaction of palldium porphyrin probe with nucleic acid

作　　者：李改茹[1] 晋卫军[2] 

机构地区：[1]新疆医科大学药学院分析药分教研室,新疆乌鲁木齐830054 [2]山西大学化学化工学院,山西太原030006

出　　处：《新疆医科大学学报》2003年第6期548-549,共2页Journal of Xinjiang Medical University

基　　金：国家自然科学基金资助项目(29875016);山西省自然科学基金资助项目资助课题(991010)

摘　　要：目的:研究水溶性钯卟啉探针与核酸的相互作用。方法:采用室温磷光探针法研究阳离子探针Pd—TAPP和阴离子探针Pd—TSPP与小牛胸腺DNA(ctDNA)的相互作用。结果:Pd-FAPP探针结合于DNA分子的浅沟槽处,缔合常数为KPd-TAPP-DNA=4.37×105L/mol。结论:Pd—TAPP与DNA分子间具有较高的亲和力,而Pd—TSPP由于带有负电荷,所以与DNA的亲和能力远弱于Pd-TAPP。Objective: To Study the interaction of water-soluble palldium porphyrin probe with nucleic acid. Methods: Room temperature phosphorescent probe method was used. Results: The probes did not intercalate in DNA but bound to its grooves. The association constant was 4. 37 × 105L/mol (b. p. ) for Pd-TAPP with ct DNA. The affinity of Pd-TSPP for cl DNA was little weaker than that of Pd-TAPP for ct DNA. Conclusion: The interactional mode of Pd-TAPP and Pd- TSPP with ct DNA was elicited in this paper.

关 键 词：室温磷光探针 钯卟啉 核酸 

分 类 号：R962[医药卫生—药理学]