外周血单个核细胞中醛糖还原酶mRNA水平与2型糖尿病患者视网膜病变的关系  

The relationship between aldose reductase mRNA levels in peripheral blood mononuclear cells and diabetic retinopathy in patients with type2diabetes mellitus

作　　者：吴芳[1] 丁伟[1] 李红[1] 何敏慧[1] 楼定华 

机构地区：[1]浙江大学医学院附属第一医院,杭州310003 [2]浙益眼科中心

出　　处：《浙江医学》2003年第12期710-712,共3页Zhejiang Medical Journal

基　　金：浙江省教育厅科研基金 (编号982013);浙江省卫生厅科研基金 (编号98A013)

摘　　要：目的 探讨外周血单个核细胞 (PBMC)中醛糖还原酶 (AR)mRNA水平与2型糖尿病患者发生视网膜病变危险性的关系。方法 运用RT-PCR技术检测54例2型糖尿病患者 (40例无视网膜病变和14例伴视网膜病变 )和35名正常对照者PBMC中ARmRNA水平。结果 2型糖尿病伴视网膜病变组PBMC中ARmRNA水平(1.07±0.94)高于正常对照组 (0.29±0.10,P<0.01)及2型糖尿病无视网膜病变组 (0.64±0.46 ,P<0.01)。糖尿病患者PBMC中ARmRNA水平与血压、血糖、血脂及病程均无显著相关。结论 PBMC中ARmRNA水平与2型糖尿病视网膜病变有密切关系。Objective To investigate the relationship between aldose reductase(AR)mRNA levels in peripheral blood mononuclear cells(PBMC)and diabetic retinopathy in patients with type2diabetes mellitus(DM).Methods A total of54patients with type2diabetes(14patients with retinopathy and40without retinopathy)were included in the study.The levels of AR mRNA in PBMC were determined by RT-PCR.Results The AR mRNA level in PBMC of diabetic patients with retinopathy(1.07±0.94)was signifiˉcantly higher than that of the patients without retinopathy(0.64±0.46,P<0.05)and the normal controls(0.29±0.10,P<0.01).Conclusion AR mRNA levels in PBMC are significantly increased in type2DM patients,especially in type2DMpatients with retinopathy.

关 键 词：外周血单个核细胞 醛糖还原酶 MRNA 2型糖尿病 视网膜病变 

分 类 号：R587.1[医药卫生—内分泌]