Covalentimmobilization of oligoDNA on the surface of magnetic nanoparticles and surface-enhanced Raman scattering study  

作　　者：SHENHeba1 WANGYoubao YANGHaifeng JIANGJisen 

机构地区：[1]DepartmentofChemistry,ShanghaiNormalUniversity,Shanghai200234,China [2]StateKeyLaboratoryofChemical&BiologicalSensorandMetric,HunanUniversity,Changsha410082,China [3]DepartmentofPhysics,CenterofFunctionalNanomaterialsandDevices,EastChinaNormalUniversity,Shanghai200062,China

出　　处：《Chinese Science Bulletin》2003年第24期2698-2702,共5页

基　　金：supported by the Natural Science Foundation of Shanghai(Grant No.02ZA14068).

摘　　要：The DNA magnetic nanoparticles are poten-tially useful in isolating and purifying DNA or RNA, direct-ing-target-medicines, the development of DNA biosensors and biochips. Surface functionalized magnetic nanoparticles with monodispersed shape and size were prepared by coating nano-sized g-Fe2O3 with silica in reverse microemulsion, and then thiol-compounds were immobilized onto the magnetic nanoparticles. After immobilizing oligoDNA modified with thiol-disulfide on the surface of the fictionalized magnetic nanoparticles, we obtained DNA-magnetic nanoparticles. The efficiency of the single-linking probes loading at the surfaces of magnetic nanoparticles was examined via hy-bridization experiment. Surface-enhanced Raman scattering methods were also effectively applied to observing the immobilization and hybridization processes mentioned above. The results demonstrated oligoDNA being availably connected to the surface of the magnetic nanoparticles.The DNA magnetic nanoparticles are poten-tially useful in isolating and purifying DNA or RNA, direct-ing-target-medicines, the development of DNA biosensors and biochips. Surface functionalized magnetic nanoparticles with monodispersed shape and size were prepared by coating nano-sized g-Fe2O3 with silica in reverse microemulsion, and then thiol-compounds were immobilized onto the magnetic nanoparticles. After immobilizing oligoDNA modified with thiol-disulfide on the surface of the fictionalized magnetic nanoparticles, we obtained DNA-magnetic nanoparticles. The efficiency of the single-linking probes loading at the surfaces of magnetic nanoparticles was examined via hy-bridization experiment. Surface-enhanced Raman scattering methods were also effectively applied to observing the immobilization and hybridization processes mentioned above. The results demonstrated oligoDNA being availably connected to the surface of the magnetic nanoparticles.

关 键 词：共价固定 DNA 磁性纳米粒子 RAMAN散射 RNA 纳米生物技术 

分 类 号：Q81[生物学—生物工程] TB383[一般工业技术—材料科学与工程]