Fatty Acid Composition Analyses of the DCMU Resistant Mutants of Nannochloropsis oculata(Eustigmatophyceae)  

作　　者：ZHANGJimin LIUShuang YANGGuanpin ZHANGXuecheng GAOZhenhui 

机构地区：[1]NorthChinaSeaMonitoringCenter,SOA,22FushunRtrut,Qingdao266033,P.R.China [2]CollegeofMarineLifeSciences,OceanUniversityofChina,Qingdao266003,P.R.China

出　　处：《Journal of Ocean University of Qingdao》2003年第1期65-68,共4页青岛海洋大学学报（英文版）

基　　金：support of the National Advanced Technology‘863’Project Foundation(819-02-01).

摘　　要：Ultraviolet mutagenesis was applied to Nannochloropsis oculata and three mutants resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) were isolated. The cellular chlorophyll a and total lipid content of the wild are higher in the medium supplemented with DCMU than in the control without DCMU. Without DCMU, the growth rates and chlorophyll a contents of the mutants are similar to those of the wild. Significant changes of fatty acid content and composition have occurred in DCMU-resistant mutants growing in the medium supplemented with DCMU. The total lipid, palmitic acid (16∶0), palmitoleic acid (16∶1ω9) and oleic (18∶1ω9) contents decrease significantly, while the vaccenic acid (18∶1ω11) increases significantly and the EPA content of dried powder increases slightly in the mutants. The study may provide a basis to improve EPA content in Nannochloropsis oculata in the future.Ultraviolet mutagenesis was applied to Nannochloropsis oculata and three mutants resistant to 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) were isolated. The cellular chlorophyll a and total lipid content of the wild are higher in the medium supplemented with DCMU than in the control without DCMU. Without DCMU, the growth rates and chlorophyll a contents of the mutants are similar to those of the wild. Significant changes of fatty acid content and composition have occurred in DCMU-resistant mutants growing in the medium supplemented with DCMU. The total lipid, palmitic acid (16∶0), palmitoleic acid (16∶1ω9) and oleic (18∶1ω9) contents decrease significantly, while the vaccenic acid (18∶1ω11) increases significantly and the EPA content of dried powder increases slightly in the mutants. The study may provide a basis to improve EPA content in Nannochloropsis oculata in the future.

关 键 词：Nannochloropsis oculata DCMU MUTATION fatty acid 

分 类 号：Q175[生物学—水生生物学]