玉米自交系幼胚离体培养及植株再生研究  

Studies on vitro culture of in-bred immuture embryos and plant regeneration of Maize(Zea mays L.)

作　　者：马莲菊[1] 高峰[2] 于翠梅[3] 刘世强[3] 

机构地区：[1]沈阳师范大学化学与生命科学院,辽宁沈阳110034 [2]中科院沈阳应用生态研究所,辽宁沈阳110016 [3]沈阳农业大学农学院,辽宁沈阳110061

出　　处：《沈阳师范大学学报（自然科学版）》2004年第1期61-64,共4页Journal of Shenyang Normal University:Natural Science Edition

基　　金：辽宁省科技厅"九五"自然科学基金资助 (96 2 330 )

摘　　要：采用 4种不同类型的玉米自交系为供试材料 ,用MS和AG培养基进行诱导培养 ,获得胚性愈伤组织 .结果表明 ,基因型和外植体的发育时期对愈伤组织的诱导率有很大影响 ;不同的2 ,4 D浓度及山梨醇浓度影响愈伤组织的保持和质量改善 ;6 BA浓度为 2mg/L时 ,有利于再分化培养 .在再分化培养中 。On MS and AG basic media,the immature embryos from 4 in-bred of maize (Zea mays L.) were induced to produce embryogenic maize calli.The results showed that genotypes and explants had an effect on the induction frequency of embryogenic calli.Different concentration of 2,4-D and Glucitol affect maintenance and improvement of embryogenic calli.The concentration with 2?mg·L~ -1 6-BA was optimal for redifferentiation culture.The plant regenerations were obtained in redifferentiation culture.

关 键 词：玉米 幼胚 植株再生 

分 类 号：S513[农业科学—作物学]