茅苍术挥发油对幽门螺杆菌及其尿素酶B表达的影响  

Effect of Atractylodes lancea DC volatile oils on Helicobacter pylori and the expression of UreB mRNA

作　　者：翟志刚[1] 余敏[2] 张开军[1] 石海波[3] 邵晨[4] 戴东方[1] 

机构地区：[1]江苏大学附属医院放疗科,江苏镇江212001 [2]江苏大学医学院病原生物学研究室,江苏镇江212013 [3]江苏大学附属医院信息科,江苏镇江212001 [4]江苏大学附属医院心内科,江苏镇江212001

出　　处：《新乡医学院学报》2016年第12期1047-1049,共3页Journal of Xinxiang Medical University

基　　金：镇江市卫生科技重点专项资助项目(编号:SHW2015001);江苏大学临床医学专项基金(编号:JDLCZX024)

摘　　要：目的研究茅苍术挥发油对幽门螺杆菌(HP)的抑制作用以及亚最小抑菌浓度(sub-MIC)挥发油对HP毒力因子尿素酶B(Ure B)表达的影响。方法将实验分为阳性对照组(加入100μL HP菌悬液),溶剂对照组(加入100μL HP菌悬液和0.25μL二甲基亚砜),30.000、15.000、7.500、3.750、1.875、0.938、0.469 g·L^(-1)茅苍术组(分别加入30.000、15.000、7.500、3.750、1.875、0.938、0.469 g·L^(-1)茅苍术挥发油和100μL HP菌悬液)和空白对照组(加入培养基),运用常规肉汤稀释法测定茅苍术挥发油对HP的抑制作用。将2×1010CFU·L^(-1)HP接种于布氏肉汤液体培养基,然后分为实验组和空白对照组,实验组加入茅苍术挥发油使其终浓度为0.5×最小抑菌浓度,对照组不加茅苍术探发油,微需氧培养72 h后,采用实时定量荧光聚合酶链式反应检测2组尿素酶B(Ure B)mRNA的表达。结果溶剂对照组和0.469 g·L^(-1)茅苍术组抑制率与阳性对照组比较差异无统计学意义(P>0.05)。30.000、15.000、7.500、3.750、1.875 g·L^(-1)茅苍术组抑制率高于阳性对照组,0.938 g·L^(-1)茅苍术组抑制率低于阳性对照组,差异均有统计学意义(P<0.05)。实验组Ure B mRNA表达量为0.036±0.001,低于对照组的0.100±0.001,差异有统计学意义(P<0.01)。结论一定浓度的茅苍术挥发油可抑制HP生长,亚抑菌浓度挥发油可下调Ure B mRNA表达。Objective To investigate the inhibitory action of the Atractylodes lancea DC volatile oils on Helicobacter pylori( HP) and the influence of volatile oils at sub-minimum inhibitory concentration( sub-MIC) on the expression of virulence factor urease B( Ure B) mRNA of HP. Methods The experiment was divided into positive control group( adding 100 μL HP suspension),solvent control group( adding 100 μL HP suspension and 0. 25 μL dimethylsulfoxide),30. 000,15. 000,7. 500,3. 750,1. 875,0. 938,0. 469 g·L^(-1)Atractylodes lancea DC group( adding 30. 000,15. 000,7. 500,3. 750,1. 875,0. 938,0. 469 g·L^(-1)Atractylodes lancea DC volatile oil and 100 μL HP suspension) and blank control group( adding medium); the broth dilution method was employed to measure the inhibitory action of Atractylodes lancea DC volatile oils on HP. The 2 ×1010CFU·L^(-1)HP was inoculated into the Brucella broth liquid medium,and then the experiment was divided into experimental group and control group. The experimental group was added Atractylodes lancea DC volatile oil to make the final concentration of HP was 0. 5 × minimum inhibitory concentration; the control group was not added Atractylodes lancea DC volatile oil; two groups was cultured under micro-aerobic condition for 72 h. The real-time polymerase chain reaction( Real-time PCR) was used to detect the expression of the Ure B mRNA in the two groups. Results There was no statistic difference in the inhibition ratio between solvent control group,0. 469 g·L^(-1)Atractylodes lancea DC group and positive control group( P > 0. 05). The inhibition ratio in 30. 000,15. 000,7. 500,3. 750,1. 875 g·L^(-1)Atractylodes lancea DC group was significantly higher than that in positive control group( P < 0. 05); the inhibition ratio in 0. 938 g·L^(-1)Atractylodes lancea DC group was significantly lower than that in positive control group( P < 0. 05). The expression of Ure B mRNA in experimental group and control group was 0. 036 ±0. 001 and 0. 100 ± 0. 001 respectively; the expression of UreB mRNA

关 键 词：茅苍术 挥发油 幽门螺杆菌 尿素酶B 

分 类 号：R285[医药卫生—中药学]