美洲大蠊不同工艺提取物对四氯化碳致急性肝损伤小鼠肝脏的保护作用  

Protective effect of Periplaneta americana extracts extracted by different technique on acute hepatic injury induced by carbon tetrachloride in mice

作　　者：周湘[1] 刘学聪[1] 张文超[1] 陈玮璨 刘昕[1] 

机构地区：[1]河北省儿童医院,河北石家庄050031

出　　处：《新乡医学院学报》2016年第12期1050-1053,共4页Journal of Xinxiang Medical University

摘　　要：目的探讨美洲大蠊不同工艺提取物对四氯化碳致急性肝损伤小鼠肝脏的保护作用。方法 72只小鼠随机分为对照组、模型组、水提取组、乙醇提取组、虫粉混悬液组和联苯双酯组,每组12只。采用腹腔注射四氯化碳法建立急性肝损伤小鼠模型。建立模型前,水提取组、乙醇提取组、虫粉混悬液组、联苯双酯组小鼠分别给予美洲大蠊水提取物、乙醇提取物、虫粉混悬液、联苯双酯灌胃,每次200 mg·kg^(-1),每日1次,连续灌胃10 d;对照组和模型组小鼠给予等量的生理盐水灌胃。建模12 h后称取小鼠体质量,麻醉下摘除眼球采血,采用酶联免疫吸附试验检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、总胆红素(TB)水平。颈椎脱臼法处死小鼠,取出肝脏并称质量,计算肝指数;制备肝脏匀浆检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)活性。结果模型组小鼠血清ALT、AST、TB水平显著高于对照组(P<0.01);水提取组、乙醇提取组、虫粉混悬液组和联苯双酯组小鼠血清ALT、AST、TB水平显著低于模型组(P<0.05);水提取组和虫粉混悬液组小鼠血清ALT、AST、TB水平显著低于乙醇提取组(P<0.05),水提取组与虫粉混悬液组小鼠血清ALT、AST、TB水平比较差异无统计学意义(P>0.05),联苯双酯组小鼠血清ALT、AST、TB水平显著低于水提取组、乙醇提取组和虫粉混悬液组(P<0.05)。模型组小鼠肝脏质量和肝脏指数均显著高于对照组(P<0.05);水提取组、乙醇提取组、虫粉混悬液组、联苯双酯组小鼠肝脏质量和肝脏指数均显著低于模型组(P<0.05);水提取组、乙醇提取组、虫粉混悬液组和联苯双酯组小鼠肝脏质量组间两两比较差异均无统计学意义(P>0.05);水提取组、乙醇提取组、虫粉混悬液组小鼠肝脏指数显著低于联苯双酯组(P<0.05),水提取组和虫粉混悬液组小鼠肝脏指数显著低于乙醇提取组(P<0.05),水提�Objective To study the protective effect of Periplaneta americana extracts extracted by different technique on acute hepatic injury induced by carbon tetrachloride（ CCl4） in mice. Methods A total of 72 mice were randomly divided into control group,model group,water extraction group,ethanol extraction group,insect powder suspension group and bifendate groups,12 mice in each group. The mouse models of acute hepatic injury were established by intraperitoneal injection of carbon tetrachloride. Before the establishment of the models,the mice in water extraction group,ethanol extraction group,insect powder suspension group and bifendate groups were treated with Periplaneta americana water extract,ethanol extract,insect powder suspension and bifendate 200 mg·kg-1by intragastric administration,once a day for ten days; the mice in control group andmodel group were treated with equal volume of normal saline by intragastric administration. The body weight of mice was weighed 12 hours after modeling. The blood was collected by enucleating the eyeball. The levels of serum alanine transarninase（ ALT）,aspartate aminotransferase（ AST） and total bilirubin（ TB） were detected by enzyme-linked immunosorbent test. The livers were enucleated and weighed after the mice were killed by cervical dislocation,then the liver index was calculated. The levels of superoxide dismutase（ SOD）,malondialdehyde（ MDA） and glutathione（ GSH） in hepatic tissue homogenate were detected. Results The levels of serum ALT,AST and TB in model group were significantly higher than those in control group（ P <0. 01）. The levels of serum ALT,AST and TB in water extraction group,ethanol extraction group,insect powder suspension group and bifendate group were significantly lower than those in model group（ P < 0. 05）. The levels of serum ALT,AST and TB in water extraction group and insect powder suspension group were significantly lower than those in ethanol extraction group（ P < 0. 05）; but there was no significant difference

关 键 词：美洲大蠊 急性肝损伤 四氯化碳 小鼠 

分 类 号：R285.5[医药卫生—中药学]