急性心肌梗死模型大鼠探索行为及海马神经元中脑源性神经营养因子表达的变化  

Changes of exploratory behavior and brain derived neurotrophic factor expression in hippocampal neurons in rats with acute myocardial infarction

作　　者：张晓鑫 王艳[1] 杨清成[2] ZHANG Xiao-xin;WANG Yan;YANG Qing-cheng(Department of Neurology,the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,Henan Province,China;Department of Neurosurgery,the People's Hospital of Anyang City,Anyang 455001,Henan Province,China)

机构地区：[1]新乡医学院第三附属医院神经内二科,河南新乡453003 [2]安阳市人民医院神经内科,河南安阳455001

出　　处：《新乡医学院学报》2019年第3期218-223,共6页Journal of Xinxiang Medical University

摘　　要：目的探讨急性心肌梗死(AMI)大鼠探索行为和海马神经元中脑源性神经营养因子(BDNF)表达的变化。方法将25只健康雄性Sprague Dawley大鼠依据分层抽样法分为对照组(n=5)和AMI组(n=20)。AMI组大鼠采用心脏左冠状动脉前降支结扎术制作AMI模型,对照组大鼠不做任何处理。采用旷场实验观察各组大鼠AMI前及AMI后3、7、10、14 d探索行为的变化,苏木精-伊红(HE)染色检测2组大鼠心肌组织变化;免疫组织化学染色法和Western blot法检测2组大鼠海马神经元中BDNF蛋白表达;实时荧光定量聚合酶链反应检测2组大鼠海马神经元中BDNF mRNA表达。结果旷场实验结果显示,AMI前2组大鼠潜伏期、水平运动次数、垂直运动次数比较差异无统计学意义(P> 0. 05); AMI后3、7、10、14 d,AMI组大鼠潜伏期长于对照组,水平运动次数、垂直运动次数少于对照组(P <0. 05)。HE染色结果显示,AMI组大鼠心肌组织梗死区及梗死边缘区的心肌细胞出现核固缩现象,且有炎细胞浸润;对照组大鼠心肌细胞呈粉红色。免疫组织化学染色和Western blot检测结果均显示,AMI后7、14 d,AMI组大鼠海马神经元中BDNF蛋白表达量低于对照组(P <0. 05); AMI组大鼠AMI后14 d海马神经元中BDNF蛋白表达量低于AMI后7 d (P <0. 05)。AMI后7、14 d,AMI组大鼠海马神经元中BDNF mRNA相对表达量低于对照组(P <0. 05); AMI后14 d,AMI组大鼠海马神经元中BDNF mRNA相对表达量低于AMI后7 d (P <0. 05)。结论大鼠AMI后伴发抑郁样行为,其机制可能与海马神经元中BDNF表达显著降低有关。Objective To investigate the changes of exploration behavior and brain derived neurotrophic factor( BDNF) expression in hippocampal neurons after acute myocardial infarction( AMI) in rats. Methods Twenty-five healthy male Sprague Dawley rats were divided into control group( n = 5) and AMI group( n = 20) according to stratified sampling.The rats in the AMI group were performed with the left anterior descending coronary artery ligation to prepare AMI model. The rats in the control group did not receive any intervention. The changes of exploration behavior of rats were observed by open field test at 0,3,7,10 and 14 days after AMI. The morphological changes of myocardial tissue of rats in the two groups were observed by hematoxylin-eosin( HE) staining. The expression of BDNF protein in hippocampal tissues of rats in the two groups was detected by immunohistochemical staining and Western blot. The expression of BDNF mRNA in the hippocampal tissues of rats in the two groups was detected by real-time quantitative polymerase chain reaction. Results The results of open field test showed that there was no statistically significant difference in the latency period,horizontal movement times and vertical movement times between the two groups before AMI( P > 0. 05). At 3,7,10 and 14 days after AMI,the latency period in the AMI group was longer than that in the control group,horizontal movement times and vertical movement times were less than those in the control group( P < 0. 05). The results of HE staining showed that the myocardial cells in the infarct area and the infarct edge area of rats in the AMI group showed nuclear shrinkage and inflammatory cell infiltration. The myocardial cells of the control group were pink. Both immunohistochemical staining and Western blot results showed that the BDNF protein expression in hippocampal neurons of rats in the AMI group was lower than that in the control group at 7 and 14 days after AMI( P < 0. 05). The BDNF protein expression in hippocampal neurons of rats in the AMI group at 14 d

关 键 词：急性心肌梗死 抑郁症 海马 神经元 脑源性神经营养因子 探索行为 

分 类 号：R542.22[医药卫生—心血管疾病] R749.4[医药卫生—内科学]