Cell behaviors on surface of pure tantalum with nano-dimpled structure  

作　　者：Jun-Wei Ma Rui Zan Wen-Zhi Chen Jia-Hua Ni Xiao-Nong Zhang 

机构地区：[1]State Key Laboratory of Metal Matrix Composites,School of Materials Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China [2]California Nanosystems Institute,University of California,Los Angeles,California,CA 90095,USA

出　　处：《Rare Metals》2019年第6期543-551,共9页稀有金属（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (Nos.51401126 and 51571142)

摘　　要：Tantalum exhibits excellent biocompatibility and good chemical stability, which has been employed in dental and orthopedic applications.The local microenvironment of the surface of implants influence determines the surrounding cell responses.In this work, nano-dimpled structure on the surface of pure tantalum was fabricated by electrochemical anodization.The surface morphology of the nano-dimpled structure was observed, and the dimensions of the nano-dimpled structure were measured by scanning electron microscopy(SEM).The surface roughness of the sample with nano-dimpled structure was measured by atomic force microscopy(AFM).The results show that the diameter of nano-dimpled ranges from 40 to 180 nm, and the higher the applied voltage, the larger the diameter.Moreover, nano-dimpled structure has good hydrophilicity.In vitro results show that nano-dimpled structure can promote the adhesion and proliferation of mouse cranial anterior bone(MC-3T3-E1) cells.The filopodia of MC-3T3-E1 cells can be fully extended on this nano-dimpled structure.On the surface of the nano-dimpled structure with a dimple size of 40 nm, the cells have the longest aspect ratio.The small dimple size of the nanodimpled structure is beneficial to cell proliferation and osteogenic differentiation.Tantalum exhibits excellent biocompatibility and good chemical stability, which has been employed in dental and orthopedic applications.The local microenvironment of the surface of implants influence determines the surrounding cell responses.In this work, nano-dimpled structure on the surface of pure tantalum was fabricated by electrochemical anodization.The surface morphology of the nano-dimpled structure was observed, and the dimensions of the nano-dimpled structure were measured by scanning electron microscopy(SEM).The surface roughness of the sample with nano-dimpled structure was measured by atomic force microscopy(AFM).The results show that the diameter of nano-dimpled ranges from 40 to 180 nm, and the higher the applied voltage, the larger the diameter.Moreover, nano-dimpled structure has good hydrophilicity.In vitro results show that nano-dimpled structure can promote the adhesion and proliferation of mouse cranial anterior bone(MC-3T3-E1) cells.The filopodia of MC-3T3-E1 cells can be fully extended on this nano-dimpled structure.On the surface of the nano-dimpled structure with a dimple size of 40 nm, the cells have the longest aspect ratio.The small dimple size of the nanodimpled structure is beneficial to cell proliferation and osteogenic differentiation.

关 键 词：TANTALUM Nano-dimpled STRUCTURE CELL BEHAVIORS Anodization 

分 类 号：R318.08[医药卫生—生物医学工程] TG146.416[医药卫生—基础医学]