复方半边莲水溶性药效部位类脂质体制备工艺优化  

Optimizing the Preparation Process of Liposomes from the Water-Soluble Pharmacodynamic Fraction of Compound Lobelia Chinensis

作　　者：王海燕 陈琼 WANG Hai-yan;CHEN Qiong(College of Biology and Pharmaceutical Engineering,Xinyang Agriculture and Forestry University,Xinyang Technical Research Center of Traditional Chinese Medicine Resources Development,Xinyang Key Laboratory for Quality Analysis and Detection of Traditional Chinese Medicine,Xinyang 464000,China)

机构地区：[1]信阳农林学院生物与制药工程学院信阳市中药资源开发工程技术研究中心信阳市中药质量分析检测重点实验室,河南信阳464000

出　　处：《信阳农林学院学报》2019年第1期104-107,共4页Journal of Xinyang Agriculture and Forestry University

基　　金：河南省科技厅科技攻关项目(编号:172102210121)

摘　　要：目的:考察影响复方半边莲水溶性药效部位类脂质体制备工艺的各因素,确定复方半边莲水溶性药效部位类脂质体最佳制备工艺条件。方法:以包封率为指标,采用正交试验设计对影响类脂质体质量的司盘-80:胆固醇、载体:药物、水合温度、水合时间进行考察。结果:复方半边莲水溶性药效部位类脂质体最佳制备工艺参数为:司盘-80:胆固醇为1:1,载体:药物为1.05:1,水合介质为PBS(pH=7.6),水合温度为60℃,超声时间10min,水合时间12min。结论:复方半边莲水溶性药效部位类脂质体制备工艺简单,稳定可行,包封率适宜。Objective:To investigate the factors affecting the preparation process of liposomes from the water-soluble pharmacodynamic fraction of compound lobelia chinensis,and determine its optimum preparation conditions.Methods:Investigated by orthogonal design with encapsulation efficiency as an index.They are respectively the ratio of Sipan 80 to cholesterol,carrier to drug,hydration temperature and hydration time.Results:The optimum preparation parameters of the liposomes of the watersoluble pharmacodynamic fraction of compound lobelia chinensis were as follows:the ratio of Sipan 80 to cholesterol was 1:1,the ratio of carrier to drug was also 1:1,the hydration medium was PBS(pH=7.6),the hydration temperature was 60℃,the ultrasonic time was 10 minutes,and the hydration time was 12 minutes.Conclusion:The preparation process of the liposomes from the water-soluble pharmacodynamics fraction of compound lobelia chinensis is simple,stable and feasible,and the encapsulation efficiency is appropriate.

关 键 词：复方半边莲 水溶性药效部位 类脂质体 制备工艺 

分 类 号：TQ461[化学工程—制药化工]