舍曲林诱导Kasumi-1细胞凋亡及其作用机制的实验研究  

Expe rime ntal Study on Apoptosis of Kasumi-1 Ce lls Induce d by Se rtraline and Its Mole cular Me chanism

作　　者：晏伟伟[1] 夏迪[1] 张颖婷[1] 潘晓蓉[1] 童建华[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院检验系上海血液学研究所医学基因组学国家重点实验室,上海200025

出　　处：《中国实验血液学杂志》2015年第4期966-970,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金(81170508;81470317);上海市领军人才"国家队"专项资金

摘　　要：目的:探讨精神类药物舍曲林对人急性髓系白血病细胞株Kasumi-1的生物学效应。方法:将不同浓度舍曲林作用于Kasumi-1细胞不同时间,通过细胞计数、细胞形态学检查、流式细胞术和Western blot分别检测舍曲林处理前后Kasumi-1细胞增殖和凋亡情况、细胞周期分布以及相关蛋白的改变。结果:舍曲林能够抑制Kasumi-1细胞增殖、诱导细胞凋亡;15和20μmol/L舍曲林处理Kasumi-1细胞24 h后,生长抑制率分别达到(19.00±7.37)%和(47.90±11.19)%;流式细胞术检测结果显示,与空白对照组相比,15和20μmol/L舍曲林处理Kasumi-1细胞24 h后,Annexin V阳性细胞百分比从(9.71±2.12)%分别上升至(20.54±2.52)%和(45.37±7.88)%(P<0.01),且细胞被明显阻滞在G0/G1期和G2/M期;此外,舍曲林还可以下调细胞内翻译控制肿瘤蛋白(translationally controlled tumor protein,TCTP)。结论:舍曲林能够显著诱导Kasumi-1细胞发生凋亡,其作用机制可能与细胞内TCTP蛋白的下调相关。Objective:To investigate the biological effects of sertraline,one of psychotropic drugs,on actue myeloid leukemia cell line Kasumi-1.Methods:Cells were treated by different concentrations of sertraline for different times.The effects of sertraline were evaluated by cell growth,cell morphology,cell cycle distribution and markers of cell apoptosis,respectively.Western blot was used to detect the expression change of related proteins.Results:Sertraline could inhibit cell proliferation and induce apoptosis.After treatment with 15 μmol/L and 20 μmol/L sertraline for 24 h,the inhibitory rate of Kasumi-1 cell proliferation was（19.00 ± 7.37）%and（47.90 ±11.19）%,respectively.Meanwhile,compared with the control group,the percentage of Annexin V positive cells in Kasumi-1 cells treated with sertraline for 24 h raised obviously from（9.71 ± 2.12）%to（20.54 ± 2.52）%and（45.37 ± 7.88）%（P<0.01）,respectively.The cells were arrested in G0/G1 and G2/M phase.In addition,it was found that sertraline could also down-regulate the level of translationally controlled tumor protein（TCTP） in Kasumi-1 cells.Conclusion:Sertraline can significantly induce the apoptosis of Kasumi-1 cells,that probably is associated with the down-regulation of TCTP protein expression.

关 键 词：舍曲林 Kasumi-1细胞株 翻译控制肿瘤蛋白 细胞凋亡 

分 类 号：R96[医药卫生—药理学]