流式微球技术检测血小板特异性抗体的研究进展  

Research Progress on Cytometric Bead Assay for Platelet Antibody Detection——Review

作　　者：凌云[1,2] 孔欣[2] 陈宝安[1] 

机构地区：[1]东南大学医学院附属中大医院血液科(江苏医学重点学科),江苏南京210009 [2]苏州大学附属第三医院常州市第一人民医院血液科,江苏常州313000

出　　处：《中国实验血液学杂志》2015年第4期1208-1211,共4页Journal of Experimental Hematology

基　　金：中国博士后科学基金面上资助(2012M511181)

摘　　要：血小板特异性抗体的产生是引起特发性血小板减少性紫癜(idiopathic thrombocytopenic purpura,ITP)病人血小板减少及巨核细胞成熟障碍的重要原因,其诊断缺乏特异性的临床表现,主要依靠血小板特异性抗体的检测。单克隆抗体特异性血小板抗原固定术(MAIPA)是检测血小板特异性抗体的"金标准",具有高特异性、低敏感性等特点,但由于操作繁琐、耗时等原因未在临床实验室推广使用。近年来,基于流式细胞术检测血小板特异性抗体的技术研究报道,其敏感性高于MAIPA,解决了部分操作及耗时的问题,并具有利用微球编码进行联立血小板抗体检测等优点。本文就近年来该检测方法的研究进展作一综述。Anti-platelet specific antibody is one of the most important reasons leading to thrombocytopenia and megakaryocyte dysmaturity. The detection of platelet autoantibodies is an important step in the diagnosis of ITP because of the absence of specific clinic feature. The monoclonal antibody-specific immobilization of platelet antigens( M AIPA)has become a ' gold standard' for determination of PLT specific antibody,which has high specificity and lowsensitivity.However,this assay is time-consuming and tedious work. Routine use of this assay in hospital is difficult. Recently,some researches reporded the cytometric bead assay that has higher sensitivity than M AIPA,and so probably solves the problem of time-consuming partly,that also can use different beads for simultaneous detection. This reviewfocuses on recent progress of the cytometric bead assay.

关 键 词：血小板特异性抗体 流式微球技术 MAIPA 

分 类 号：R446.6[医药卫生—诊断学]