不同贮存时间的悬浮红细胞中精氨酸酶水平的研究  

Arginase Level in Suspended Red Blood Cells Storaged for Different Time

作　　者：范丽萍[1,2] 黄豪博[1,2] 魏世金 傅丹晖[1,3,2] 曾丰[1,2] 黄清华[1,2] 洪金全 

机构地区：[1]福建医科大学附属协和医院输血科福建省血液病研究所,福建福州350001 [2]福建省临床用血质量控制中心,福建福州350001 [3]福建医科大学附属协和医院血液科福建省血液病研究所,福建福州350001 [4]福建医科大学附属第二医院麻醉科,福建泉州362000

出　　处：《中国实验血液学杂志》2015年第5期1459-1463,共5页Journal of Experimental Hematology

基　　金：福建省卫生厅青年科研课题(编号:2011-1-12);泉州市科技项目B类(编号:15)

摘　　要：目的:探讨不同贮存时间对悬浮红细胞中精氨酸酶水平的影响及悬浮红细胞中精氨酸酶的可能来源。方法:应用ELISA法检测不同贮存时间悬浮红细胞和对照血浆中精氨酸酶水平及髓过氧化物酶(MPO)的水平;比色法检测不同贮存时间悬浮红细胞和对照血浆中游离血红蛋白水平;应用相关性分析分析方法不同贮存时间悬浮红细胞中精氨酸酶水平与游离血红蛋白水平及MPO水平的相关性。结果:不同贮存时间的悬浮红细胞中精氨酸酶、游离血红蛋白水平均显著高于对照血浆;但是,不同贮存时间的悬浮红细胞与对照血浆中MPO水平差异无显著性。三者均不随贮存时间的延长而增加。不同贮存时间的悬浮红细胞中精氨酸酶水平与游离血红蛋白水平不相关(r=0.03),但与MPO水平呈显著正相关(r=0.76)。结论:不同贮存时间的悬浮红细胞中精氨酸酶水平显著升高,但不随贮存时间延长而增加。悬浮红细胞中残留的少量白细胞,特别是中性粒细胞,可能是精氨酸酶的主要来源。Objective: To explore the effect of storage time on arginase level,and the possible source of arginase in suspended red blood cells( RBC). Methods: The arginase and myeloperoxidase( MPO) levels in suspended RBC and control plasma were detected by ELISA. The free hemoglobin level in suspended RBC and control plasma were detected by colorimetric method. The relationship between arginase level,MPO level and free hemoglobin level in suspended RBC was analyzed by the related methods. Results: The arginase and free hemoglobin levels in suspended RBC were higher than those in control plasma. Otherwise,MPO level was not significantly different between suspended RBC and control plasma. All of them did not increase along with prolonging of storage time. There was not a significant correlation between arginase level and free hemoglobin level in suspended RBC of different storage time( r = 0. 03),but arginase level positively correlated with MPO level in the suspended RBC of different storage time( r = 0. 76). Conclusion: The arginase level in suspended RBC storaged for different time increases significantly,but not along with prolonging of storage time.The main possible source of arginase in the suspended RBC is the residual white blood cell,especially neutrophils.

关 键 词：悬浮红细胞 贮存时间 精氨酸酶 游离血红蛋白 髓过氧化物酶 

分 类 号：R457.1[医药卫生—治疗学]