Partial Sequence Analysis of Mitochondrial COI Gene of the Chinese Shrimp,Fenneropenaeus Chinensis  

作　　者：GAOTianxiang LIJian WANGQingyin LIUJinxian 

机构地区：[1]TheKeyLaboratoryofMariculture,MinistryofEducation,OceanUniversityofChina,Qingdao266003,P.R.China [2]YellowSeaFisheriesResearchInstitute,ChineseAcademyofFisherySciences,Qingdao266071,P.R.China

出　　处：《Journal of Ocean University of Qingdao》2003年第2期167-170,共4页青岛海洋大学学报（英文版）

基　　金：funded by Projects under the Major State Basic Research Development Program(G1999012009).

摘　　要：Eight hundred and thirty eight base pair fragment of mitochondrial COI gene of wild and cultured populations (CP1, CP4, CP5 and CP6) of Fenneropenaeus chinensis was amplified and sequenced. The A, T, G and C contents of the sequence were 235bp(28.0%), 307bp(36.6%), 138bp(16.5%) and 158bp(18.9%), respectively. Furthermore, 556bp fragment of the sequence was used to discuss the phylogenetic relationship among 14 Penaeidae species using Alpheus armillatus as the outgroup. From the molecular phylogenetic tree constructed by neighbor-joining method, we obtained three large shrimp groups:Farfantepenaeus, Litopenaeus and Fenneropenaeus group. The results also indicated that there were a closer genetic relationships between F. aztecus and F. paulensis, L. schmitti and L. setiferus, F. indicus and F. merguiensis, and the genus Farfantepenaeus was closer to Litopenaeus.Eight hundred and thirty eight base pair fragment of mitochondrial COI gene of wild and cultured populations (CP1, CP4, CP5 and CP6) of Fenneropenaeus chinensis was amplified and sequenced. The A, T, G and C contents of the sequence were 235bp (28.0% ),.307bp (36.6%), 138bp (16.5%) and 158bp (18.9%), respectively. Furthermore, 556bp fragment of the sequence was used to discuss the phylogenetic relationship among 14 Penaeidae species using Alpheus armillatus as the outgroup. From the molecular phylogenetic tree constructed by neighbor-joining method, we obtained three large shrimp groups: Farfantepenaeus, Litopenaeus and Fenneropenaeus group. The results also indicated that there were a closer genetic relationships between F. aztecus and F. paulensis, L. schmitti and L. setiferus, F. indicus and F. merguiensis, and the genus Farfantepenaeus was closer to Litopenaeus.

关 键 词：Fenneropenaeus chinensis cytochrome oxidase subunit 1(COI) DNA sequence PENAEIDAE phylogeny 

分 类 号：Q953[生物学—动物学]