化湿利水泄浊法对自发性高血压大鼠主动脉血管结构的影响  

Influence of Dampness-Transforming,Water-Disinhibiting and Turbidity-Draining Therapy on the Structure of Aortic Arteries in Spontaneous Hypertensive Rats

作　　者：何立人[1] 王世君[1] 钱义明[1] 陈长勋[2] 陶建生[2] 张庆荣[1] 

机构地区：[1]上海中医药大学附属岳阳医院,上海200437 [2]上海中医药大学中药学院,上海200032

出　　处：《上海中医药大学学报》2003年第4期45-48,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：上海市教委博士点基金资助项目

摘　　要：为观察化湿利水泄浊法 (DTM )对自发性高血压大鼠 (SHR)主动脉血管结构的影响。 2 7只 12周龄SHR ,随机分为模型组 (B)、化湿利水泄浊组 (C)、依那普利组 (D) ,另选同周龄大鼠 9只作为正常对照组 (A) ,灌胃给药 8周。测量清醒状态下尾动脉收缩压 ,及血浆内皮素 (ET)、降钙基因相关肽 (CGRP) ,血管壁厚度 /管外径 (WT/LD)、管壁面积/管面积 (MA/WA)、管腔面积 /管面积 (LA/WA) ,主动脉血管平滑肌细胞增殖核抗原 (PCNA)的表达。结果 :与A组比较 ,收缩压B组明显升高 ,C组明显下降 ;B组ET浓度升高 ,C组下降 ;B组主动脉超微结构见中膜纤维化改变 ,细胞间隙增宽 ,平滑肌细胞排列紊乱等改变 ;C、D组动脉超微结构改变较轻 ;B组平滑肌细胞PCNA表达明显增强 ,C、D组降低。提示化湿利水泄浊法能部分逆转SHR主动脉的超微结构改变 。To observe the influence of dampness-transforming,water-disinhibiting and turbidity-draining therapy on the structure of aortic arteries in spontaneous hypertensive rats,27 spontaneous hypertensive rats(SHR)of twelve weeks were divided into three groups randomly:group B,group C and group D,and another 9 rats were used as control group(group A).The blood pressure(BP)of tail artery was measured;the concentrations of plasma calcitonin gene-related peptide(CGRP)and endothelin(ET)were measured by specific radioimmunoassay methods;WT/LD,MA/WA,LA/WA of aortic arteries were calculated by computer image analysis system.Results:Compared with group A,the BP was higher in group B(P<0.05),while it decreased in group C;ET level was higher in group B,While it decreased in group C(P<0.05);compared with group B,there was no significant change of WT/LD,LA/WA and MA/WA in group C (P>0.05);the media had severe fibrosis change,disordering array in VSMC,widening of the intercellular space in group B;the microstructural changes in groups C and D were light;compared with group A,the positive area ratio of PCNA in VSMC was increased (P<0.05);compared with group B,the positive area ratio of PCNA was decreased in groups C and D (P<0.01).it is suggested that this therapy can effectively reverse aortic arteries structure changes possibly by inhibiting the proliferation of VSMC.

关 键 词：自发性高血压 化湿利水泄浊法 中医药疗法 主动脉 血管结构 

分 类 号：R259.4[医药卫生—中西医结合]